Product Details

Purity

>85% (SDS-PAGE)

Target Names

ZBTB7A

Uniprot No.

O95365

Alternative Names

Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI 1; FBI-1; FBI1; HIV 1 1st binding protein 1; HIV-1 1st-binding protein 1; Leukemia/lymphoma related factor; Leukemia/lymphoma-related factor; LRF; POK erythroid myeloid ontogenic factor; Pokemon; POZ and Krueppel erythroid myeloid ontogenic factor; TIP21; TTF I interacting peptide 21; TTF-I-interacting peptide 21; ZBT7A_HUMAN; ZBTB7; ZBTB7A; Zinc finger and BTB domain containing protein 7A; Zinc finger and BTB domain-containing protein 7A; Zinc finger protein 857A

Species

Homo sapiens (Human)

Expression Region

1-584

Target Protein Sequence

MAGGVDGPIG IPFPDHSSDI LSGLNEQRTQ GLLCDVVILV EGREFPTHRS VLAACSQYFK KLFTSGAVVD QQNVYEIDFV SAEALTALMD FAYTATLTVS TANVGDILSA ARLLEIPAVS HVCADLLDRQ ILAADAGADA GQLDLVDQID QRNLLRAKEY LEFFQSNPMN SLPPAAAAAA ASFPWSAFGA SDDDLDATKE AVAAAVAAVA AGDCNGLDFY GPGPPAERPP TGDGDEGDSN PGLWPERDED APTGGLFPPP VAPPAATQNG HYGRGGEEEA ASLSEAAPEP GDSPGFLSGA AEGEDGDGPD VDGLAASTLL QQMMSSVGRA GAAAGDSDEE SRADDKGVMD YYLKYFSGAH DGDVYPAWSQ KVEKKIRAKA FQKCPICEKV IQGAGKLPRH IRTHTGEKPY ECNICKVRFT RQDKLKVHMR KHTGEKPYLC QQCGAAFAHN YDLKNHMRVH TGLRPYQCDS CCKTFVRSDH LHRHLKKDGC NGVPSRRGRK PRVRGGAPDP SPGATATPGA PAQPSSPDAR RNGQEKHFKD EDEDEDVASP DGLGRLNVAG AGGGGDSGGG PGAATDGNFT AGLA

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation. Directly and specifically binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions. Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms. That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300. Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors. Also directly interacts with transcription factors like SP1 to prevent their binding to DNA. Functions as an androgen receptor/AR transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements/ARE on target genes. Thereby, negatively regulates androgen receptor signaling and androgen-induced cell proliferation. Involved in the switch between fetal and adult globin expression during erythroid cells maturation. Through its interaction with the NuRD complex regulates chromatin at the fetal globin genes to repress their transcription. Specifically represses the transcription of the tumor suppressor ARF isoform from the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation. Regulates chondrogenesis through the transcriptional repression of specific genes via a mechanism that also requires histone deacetylation. Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis. Involved in adipogenesis through the regulation of genes involved in adipocyte differentiation. Plays a key role in the differentiation of lymphoid progenitors into B and T lineages. Promotes differentiation towards the B lineage by inhibiting the T-cell instructive Notch signaling pathway through the specific transcriptional repression of Notch downstream target genes. Also regulates osteoclast differentiation. May also play a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining/cNHEJ. Recruited to double-strand break sites on damage DNA, interacts with the DNA-dependent protein kinase complex and directly regulates its stability and activity in DNA repair. May also modulate the splicing activity of KHDRBS1 toward BCL2L1 in a mechanism which is histone deacetylase-dependent and thereby negatively regulates the pro-apoptotic effect of KHDRBS1.

Gene References into Functions

provide the evidence for the regulatory role of miR-106b in HCC, which is causally linked to targeting of Zbtb7a PMID: 29975452
The specific association of ZBTB7A mutations with t(8;21) rearranged acute myeloid leukaemia points towards leukemogenic cooperativity between mutant ZBTB7A and the RUNX1/RUNX1T1 fusion protein has been reported. PMID: 27252013
ZBTB7A is increased in cisplatin-resistant osteosarcoma cells and elevated ZBTB7A inhibits cisplatin-induced apoptosis by repressing LINC00473 expression. PMID: 28942243
Our data suggest that ZBTB7A is involved in the development and progression of colorectal carcinoma PMID: 29715100
Results indicate the reverse association between the expression of ZBTB7A and the tumorigenicity of nasopharyngeal carcinoma (NPC). PMID: 29699474
Alveolar macrophages deficient in Zbtb7a failed to induce antibody and T cell responses. PMID: 28701473
Variants of ZBTB7A (LRF) and its beta-globin gene cluster binding motifs in sickle cell anemia/ PMID: 27282567
This analysis complements the understanding of the mutational landscape of core binding factor acute myeloid leukemia, and has led to the identification of novel ZBTB7A mutations in t(8;21) acute myeloid leukemia subgroup. PMID: 26968532
Lymphoma-related factor mediates the protease-activated receptor 2-suppressed p53 expression in the cancer cells and binds DAP5 to interfere with the gene transcription of p53 in colon cancer cells. PMID: 28849590
Our study uncovers the loss-of-function mutation in ZBTB7A as a novel mechanism causing elevated glycolysis in human cancer, which carries important therapeutic implication. PMID: 26455326
Mutation in ZBTB7A is associated with core-binding factor acute myeloid leukemia. PMID: 27798625
Zbtb7a knockdown by siRNA suppressed cell proliferation and induced G1 cell cycle arrest and apoptosis in lung cancer cells. Furthermore, miR-125a expression showed a negative correlation with Zbtb7a expression in non-small cell lung cancer tissues. PMID: 26713860
Data suggest that microRNA miR-520e directly targets to the 3'-untranslated region (3'-UTR) of zinc finger and BTB domain containing 7A protein (Zbtb7a). PMID: 28242196
CCAT2 promotes tumorigenesis by over-expression of Pokemon. PMID: 28088736
High FBI-1 expression is associated with hepatocellular carcinoma. PMID: 27492460
there is an auto-regulatory circuit consisting of miR-125 and Pokemon, which promotes the progression of HCC and may be a promising therapeutic target in clinical HCC treatment PMID: 26227218
Pokemon was found to enhance the migration and invasion of hepatocellular carcinoma by increasing MEF2D expression PMID: 26797719
Pokemon was significantly downregulated in OSCC. PMID: 25439053
ZBTB7A directly binds to the promoter and transcriptionally represses the expression of MCAM, establishing ZBTB7A as a bona fide transcriptional repressor of MCAM PMID: 25995384
MEF2D was also found to increase the transcription of Pokemon by binding myocyte enhancer factor 2 (MEF2) sites within its promoter region, that can promote Hepatocellular carcinoma invasion PMID: 26164003
this study found that LRF/ZBTB7A transcription factor occupies fetal gamma-globin genes and maintains the nucleosome density necessary for gamma-globin gene silencing in adults, and that LRF confers its repressive activity through a NuRD repressor complex independent of the fetal globin repressor BCL11A. PMID: 26816381
p38beta is a novel regulatory target of the transcription factor Pokemon and positively regulated by Pokemon in hepatic cells. PMID: 23807508
Taken together, all these data suggest that Pokemon is a new partner of Smad4 and plays a negative role in TGF-beta pathway. PMID: 25514493
Pokemon enhanced the oncogenesis of colorectal cancer by promoting cell proliferation, cell cycle progression and anti-apoptosis activity. PMID: 25367850
our findings suggest that TGF-b1 may inhibit the expression of Pokemon, b-catenin, and E-cadherin. The high expression of TGF-b1 leads to an increase in the phenotype and apical-base polarity of epithelial cells PMID: 25722217
a potential regulatory role of FBI-1 in transcription factor activity of ETS-1 PMID: 24857950
Pokemon expression of NP69 and C666-1 obviously varied from mRNA to protein. PMID: 24377524
The mRNA expression level of Pokemon in colorectal adenocarcinoma was significantly higher than that in adjacent tumor specimens. The positive expression ratio of Pokemon protein in colorectal cancer was significantly higher than in the adjacent tissues. PMID: 24175766
An unexpected function for FBI-1 in splicing modulation with a direct impact on cell survival. PMID: 24514149
ZBTB7A, a member of the POK (POZ/BTB and Kruppel) transcription repressor family, directly binds to the promoter and represses the transcription of critical glycolytic genes, including GLUT3, PFKP, and PKM PMID: 25184678
For the first time, we show that LRF/Pokemon is predominantly expressed in nodular lymphocyte-predominant Hodgkin lymphoma with low to absent NOTCH1 protein expression PMID: 24326827
Investigated the functions of Pokemon in the carcinogenesis and development of bladder cancer and observed the inhibitory effects of siRNA expression vector on Pokemon in bladder cancer cells. PMID: 24393848
Pokemon might serve as an important mediator of crosstalk between intrinsic and extrinsic apoptotic pathways in HCC cel PMID: 23874836
Data indicate that ZBTB7A is identified as a strong candidate for regulation of thymic insulin expression. PMID: 23911422
our results may help better understand the role of Pokemon in hepatocellular carcinoma and promote the clinical applications PMID: 24261083
Knockdown of Pokemon protein expression inhibits hepatocellular carcinoma cell proliferation by suppression of AKT activity. PMID: 23924858
ZBTB7A is a context-dependent cancer gene that can act as an oncogene in some contexts but also has oncosuppressive-like activity in PTEN-null tumors. PMID: 23727861
Pokemon promotes cell proliferation and migration in hepatocellular carcinoma and accelerates tumor development. PMID: 23300578
Studies indicate that LRF regulates hematopoiesis by playing specific roles in different cell lineages. PMID: 23396304
Pokemon activates the expression of both p65 and IkappaBalpha by sequence-specific binding to their promoters and plays a dual role in regulating NF-kappaB signaling. PMID: 23054188
The oncogene LRF is a survival factor in chondrosarcoma and contributes to tumor malignancy and drug resistance. PMID: 22847180
The activities of CDK2 and E2F4 promoters were suppressed by the modulation of Zbtb7 levels and that Zbtb7 represses promoter activities through a mechanism involving direct binding of Zbtb7 to the promoters. PMID: 22447046
Pokemon promotes breast cancer progression by upregulating survivin expression and may be a potential target for the treatment of this malignancy PMID: 21392388
Depletion of Pokemon inhibited proliferation of HepG(2) or induced apoptosis. PMID: 21985851
The current findings indicated that FBI-1 plays an important role in HCC carcinogenesis and chemotherapy tolerance PMID: 21713761
siRNA of Pokemon was applied to inhibit the expression of Pokemon and NF-kappa B p65 and apoptotic rate was determined by flow cytometric analysis PMID: 21771706
The aim of this study was to examine the regulation of LRF expression in human prostate cells. PMID: 21640721
FBI-1/OCZF/LRF regulates osteoclast formation and apoptosis in vivo, and may become a useful marker and target in treating disorders leading to reduced bone density, including chronic arthritis. PMID: 21590684
FBI-1 functions as a novel androgen receptor co-repressor in prostate cancer cells. PMID: 20812024
The expression of LRF was evaluated in benign prostate hyperplastic (BPH) and prostate cancer (PC) tissues. PMID: 21251909

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Subcellular Location

Nucleus.

Tissue Specificity

Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas,

Database Links

HGNC: 18078

OMIM: 605878

KEGG: hsa:51341

STRING: 9606.ENSP00000323670

UniGene: Hs.591384

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Code	CSB-YP026343HU
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP026343HU
MSDS	MSDS
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Source	E.coli
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Code	CSB-EP026343HU-B
MSDS	MSDS
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Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP026343HU
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Source	Baculovirus
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Code	CSB-MP026343HU
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Source	Mammalian cell
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Recombinant Human Zinc finger and BTB domain-containing protein 7A (ZBTB7A)

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