Product Details

Uniprot No.

Target Names

Alternative Names

Zinc finger and BTB domain-containing protein 7A (Factor binding IST protein 1) (FBI-1) (Factor that binds to inducer of short transcripts protein 1) (HIV-1 1st-binding protein 1) (Leukemia/lymphoma-related factor) (POZ and Krueppel erythroid myeloid ontogenic factor) (POK erythroid myeloid ontogenic factor) (Pokemon) (TTF-I-interacting peptide 21) (TIP21) (Zinc finger protein 857A), ZBTB7A, FBI1 LRF ZBTB7 ZNF857A

Species Reactivity

Human

Immunogen

A synthesized peptide from human ZBTB7A protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8D5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ZBTB7A, also known as LRF (leukemia/lymphoma-related factor) or Pokemon, is a transcriptional repressor belonging to the POK family of zinc finger proteins. This protein plays critical roles in cellular differentiation, lineage commitment, and oncogenesis, functioning as a master regulator in hematopoietic development and serving as a proto-oncogene in various malignancies. Its involvement in repressing tumor suppressor genes and regulating metabolic pathways makes ZBTB7A a significant target for cancer biology and developmental research.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human ZBTB7A, offers the consistency and reproducibility that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies.

Validation across multiple platforms demonstrates this antibody's versatility for diverse experimental approaches. Western blot analysis confirms specific detection in numerous human cell lines including A431, MCF-7, HT-29, THP-1, HEK293, HeLa, and A549, with an observed band at approximately 70 kDa. This migration slightly above the predicted 61 kDa molecular weight likely reflects post-translational modifications such as phosphorylation or glycosylation common to transcription factors. Immunohistochemistry validation in human colorectal cancer and small intestine tissues demonstrates clear nuclear staining patterns consistent with ZBTB7A's function as a transcription factor. Additional validation includes immunofluorescence microscopy in MCF-7 cells and flow cytometry analysis in BxPC-3 cells, providing flexibility for both imaging and quantitative applications.

This antibody serves researchers investigating transcriptional regulation, hematopoiesis, metabolic reprogramming, and cancer biology where ZBTB7A function is implicated.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation. Directly and specifically binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions. Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms. That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300. Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors. Also directly interacts with transcription factors like SP1 to prevent their binding to DNA. Functions as an androgen receptor/AR transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements/ARE on target genes. Thereby, negatively regulates androgen receptor signaling and androgen-induced cell proliferation. Involved in the switch between fetal and adult globin expression during erythroid cells maturation. Through its interaction with the NuRD complex regulates chromatin at the fetal globin genes to repress their transcription. Specifically represses the transcription of the tumor suppressor ARF isoform from the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation. Regulates chondrogenesis through the transcriptional repression of specific genes via a mechanism that also requires histone deacetylation. Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis. Involved in adipogenesis through the regulation of genes involved in adipocyte differentiation. Plays a key role in the differentiation of lymphoid progenitors into B and T lineages. Promotes differentiation towards the B lineage by inhibiting the T-cell instructive Notch signaling pathway through the specific transcriptional repression of Notch downstream target genes. Also regulates osteoclast differentiation. May also play a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining/cNHEJ. Recruited to double-strand break sites on damage DNA, interacts with the DNA-dependent protein kinase complex and directly regulates its stability and activity in DNA repair. May also modulate the splicing activity of KHDRBS1 toward BCL2L1 in a mechanism which is histone deacetylase-dependent and thereby negatively regulates the pro-apoptotic effect of KHDRBS1.

Gene References into Functions

provide the evidence for the regulatory role of miR-106b in HCC, which is causally linked to targeting of Zbtb7a PMID: 29975452
The specific association of ZBTB7A mutations with t(8;21) rearranged acute myeloid leukaemia points towards leukemogenic cooperativity between mutant ZBTB7A and the RUNX1/RUNX1T1 fusion protein has been reported. PMID: 27252013
ZBTB7A is increased in cisplatin-resistant osteosarcoma cells and elevated ZBTB7A inhibits cisplatin-induced apoptosis by repressing LINC00473 expression. PMID: 28942243
Our data suggest that ZBTB7A is involved in the development and progression of colorectal carcinoma PMID: 29715100
Results indicate the reverse association between the expression of ZBTB7A and the tumorigenicity of nasopharyngeal carcinoma (NPC). PMID: 29699474
Alveolar macrophages deficient in Zbtb7a failed to induce antibody and T cell responses. PMID: 28701473
Variants of ZBTB7A (LRF) and its beta-globin gene cluster binding motifs in sickle cell anemia/ PMID: 27282567
This analysis complements the understanding of the mutational landscape of core binding factor acute myeloid leukemia, and has led to the identification of novel ZBTB7A mutations in t(8;21) acute myeloid leukemia subgroup. PMID: 26968532
Lymphoma-related factor mediates the protease-activated receptor 2-suppressed p53 expression in the cancer cells and binds DAP5 to interfere with the gene transcription of p53 in colon cancer cells. PMID: 28849590
Our study uncovers the loss-of-function mutation in ZBTB7A as a novel mechanism causing elevated glycolysis in human cancer, which carries important therapeutic implication. PMID: 26455326
Mutation in ZBTB7A is associated with core-binding factor acute myeloid leukemia. PMID: 27798625
Zbtb7a knockdown by siRNA suppressed cell proliferation and induced G1 cell cycle arrest and apoptosis in lung cancer cells. Furthermore, miR-125a expression showed a negative correlation with Zbtb7a expression in non-small cell lung cancer tissues. PMID: 26713860
Data suggest that microRNA miR-520e directly targets to the 3'-untranslated region (3'-UTR) of zinc finger and BTB domain containing 7A protein (Zbtb7a). PMID: 28242196
CCAT2 promotes tumorigenesis by over-expression of Pokemon. PMID: 28088736
High FBI-1 expression is associated with hepatocellular carcinoma. PMID: 27492460
there is an auto-regulatory circuit consisting of miR-125 and Pokemon, which promotes the progression of HCC and may be a promising therapeutic target in clinical HCC treatment PMID: 26227218
Pokemon was found to enhance the migration and invasion of hepatocellular carcinoma by increasing MEF2D expression PMID: 26797719
Pokemon was significantly downregulated in OSCC. PMID: 25439053
ZBTB7A directly binds to the promoter and transcriptionally represses the expression of MCAM, establishing ZBTB7A as a bona fide transcriptional repressor of MCAM PMID: 25995384
MEF2D was also found to increase the transcription of Pokemon by binding myocyte enhancer factor 2 (MEF2) sites within its promoter region, that can promote Hepatocellular carcinoma invasion PMID: 26164003
this study found that LRF/ZBTB7A transcription factor occupies fetal gamma-globin genes and maintains the nucleosome density necessary for gamma-globin gene silencing in adults, and that LRF confers its repressive activity through a NuRD repressor complex independent of the fetal globin repressor BCL11A. PMID: 26816381
p38beta is a novel regulatory target of the transcription factor Pokemon and positively regulated by Pokemon in hepatic cells. PMID: 23807508
Taken together, all these data suggest that Pokemon is a new partner of Smad4 and plays a negative role in TGF-beta pathway. PMID: 25514493
Pokemon enhanced the oncogenesis of colorectal cancer by promoting cell proliferation, cell cycle progression and anti-apoptosis activity. PMID: 25367850
our findings suggest that TGF-b1 may inhibit the expression of Pokemon, b-catenin, and E-cadherin. The high expression of TGF-b1 leads to an increase in the phenotype and apical-base polarity of epithelial cells PMID: 25722217
a potential regulatory role of FBI-1 in transcription factor activity of ETS-1 PMID: 24857950
Pokemon expression of NP69 and C666-1 obviously varied from mRNA to protein. PMID: 24377524
The mRNA expression level of Pokemon in colorectal adenocarcinoma was significantly higher than that in adjacent tumor specimens. The positive expression ratio of Pokemon protein in colorectal cancer was significantly higher than in the adjacent tissues. PMID: 24175766
An unexpected function for FBI-1 in splicing modulation with a direct impact on cell survival. PMID: 24514149
ZBTB7A, a member of the POK (POZ/BTB and Kruppel) transcription repressor family, directly binds to the promoter and represses the transcription of critical glycolytic genes, including GLUT3, PFKP, and PKM PMID: 25184678
For the first time, we show that LRF/Pokemon is predominantly expressed in nodular lymphocyte-predominant Hodgkin lymphoma with low to absent NOTCH1 protein expression PMID: 24326827
Investigated the functions of Pokemon in the carcinogenesis and development of bladder cancer and observed the inhibitory effects of siRNA expression vector on Pokemon in bladder cancer cells. PMID: 24393848
Pokemon might serve as an important mediator of crosstalk between intrinsic and extrinsic apoptotic pathways in HCC cel PMID: 23874836
Data indicate that ZBTB7A is identified as a strong candidate for regulation of thymic insulin expression. PMID: 23911422
our results may help better understand the role of Pokemon in hepatocellular carcinoma and promote the clinical applications PMID: 24261083
Knockdown of Pokemon protein expression inhibits hepatocellular carcinoma cell proliferation by suppression of AKT activity. PMID: 23924858
ZBTB7A is a context-dependent cancer gene that can act as an oncogene in some contexts but also has oncosuppressive-like activity in PTEN-null tumors. PMID: 23727861
Pokemon promotes cell proliferation and migration in hepatocellular carcinoma and accelerates tumor development. PMID: 23300578
Studies indicate that LRF regulates hematopoiesis by playing specific roles in different cell lineages. PMID: 23396304
Pokemon activates the expression of both p65 and IkappaBalpha by sequence-specific binding to their promoters and plays a dual role in regulating NF-kappaB signaling. PMID: 23054188
The oncogene LRF is a survival factor in chondrosarcoma and contributes to tumor malignancy and drug resistance. PMID: 22847180
The activities of CDK2 and E2F4 promoters were suppressed by the modulation of Zbtb7 levels and that Zbtb7 represses promoter activities through a mechanism involving direct binding of Zbtb7 to the promoters. PMID: 22447046
Pokemon promotes breast cancer progression by upregulating survivin expression and may be a potential target for the treatment of this malignancy PMID: 21392388
Depletion of Pokemon inhibited proliferation of HepG(2) or induced apoptosis. PMID: 21985851
The current findings indicated that FBI-1 plays an important role in HCC carcinogenesis and chemotherapy tolerance PMID: 21713761
siRNA of Pokemon was applied to inhibit the expression of Pokemon and NF-kappa B p65 and apoptotic rate was determined by flow cytometric analysis PMID: 21771706
The aim of this study was to examine the regulation of LRF expression in human prostate cells. PMID: 21640721
FBI-1/OCZF/LRF regulates osteoclast formation and apoptosis in vivo, and may become a useful marker and target in treating disorders leading to reduced bone density, including chronic arthritis. PMID: 21590684
FBI-1 functions as a novel androgen receptor co-repressor in prostate cancer cells. PMID: 20812024
The expression of LRF was evaluated in benign prostate hyperplastic (BPH) and prostate cancer (PC) tissues. PMID: 21251909

Hide All

Subcellular Location

Nucleus.

Tissue Specificity

Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas,

Database Links

HGNC: 18078

OMIM: 605878

KEGG: hsa:51341

STRING: 9606.ENSP00000323670

UniGene: Hs.591384

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Code	CSB-RA063930A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A431 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), HT-29 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), HeLa whole cell lysate(30µg), A549 whole cell lysate(30µg) All lanes: ZBTB7A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 61 kDa Observed band size: 70 kDa Exposure time：2min IHC image of CSB-RA063930A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA063930A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of MCF-7 cell with CSB-RA063930A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing BxPC-3 cells stained with CSB-RA063930A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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ZBTB7A Recombinant Monoclonal Antibody

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