Recombinant Human cGMP-inhibited 3',5'-cyclic phosphodiesterase A(PDE3A) ,partial

Code CSB-YP614528HU
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Source Yeast
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Code CSB-EP614528HU
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Source E.coli
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Code CSB-EP614528HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP614528HU
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Source Baculovirus
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Code CSB-MP614528HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names PDE3A
Uniprot No. Q14432
Alternative Names 5''-cyclic phosphodiesterase A; cAMP phosphodiesterase myocardial cGMP inhibited; CGI PDE ; CGI PDE A; CGI-PDE A; cGMP inhibited 3' 5' cyclic phosphodiesterase A; cGMP-inhibited 3''; Cyclic GMP inhibited phosphodiesterase A; Cyclic GMP-inhibited phosphodiesterase A; Human cGMP inhibited cAMP phosphodiesterase mRNA; PDE 3A; Pde3a; PDE3A_HUMAN; Phosphodiesterase 3A; Phosphodiesterase 3A cGMP inhibited
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
Gene References into Functions
  1. PDE3A is downregulated in chemoresistant NSCLC cells due to DNA hypermethylation. PMID: 28678321
  2. Vulnerability to antineoplastic phosphodiesterase inhibitors inhibitors has been associated with co-expression of PDE3A and SLFN12 in various tumor types. PMID: 29107068
  3. We found a novel heterozygous missense mutation c.1336T>C in exon 4 of the PDE3A gene in a Japanese family with multiple HTNB patients. This mutation was found to be completely linked to the family members who inherited this condition. PMID: 27053290
  4. The genetic variant, rs3794271, located within the PDE3A-SLCO1C1 locus was analyzed for correlation with treatment response using both the EULAR classification criteria and absolute change in (Delta)DAS28 scores as outcome measures correlated with anti-TNF response in a large UK rheumatoid arthritis cohort. PMID: 27180831
  5. PDE3A is a low-affinity and high-velocity enzyme for hydrolysis of cUMP in cardiac myocytes. PMID: 27975297
  6. Chemical proteomics results show that besides known interactors such as 14-3-3 family proteins, PDE3A was found to associate with a PP2A complex composed of a regulatory, scaffold and catalytic subunit. PMID: 26205238
  7. We found a highly significant association between PDE3A-SLCO1C1 and the clinical response to TNF blockers PMID: 25403996
  8. A heterozygous missense mutation in PDE3A causes autosomal dominant hypertension with brachydactyly. PMID: 25961942
  9. PDE3A is part of a SERCA2 signaling complex in cardiac myocytes. PMID: 25593322
  10. the genotypic and allelic frequencies of the rs7134375 SNP are different between the Mulao and Han populations. the PDE3A rs7134375 SNP is associated with serum TG levels in the Mulao population, however, not in the Han population. PMID: 24604378
  11. The PDE3A-SLCO1C1 locus rs3794271 SNP showed a highly significant association with anti-TNF treatment response. PMID: 23651021
  12. PI3Kgamma coordinates the coincident signaling of the major cardiac PDE3 and PDE4 isoforms, thus orchestrating a feedback loop that prevents calcium-dependent ventricular arrhythmia. PMID: 23008439
  13. Anagrelide suppresses megakaryocytopoiesis by reducing the expression levels of GATA-1 and FOG-1 via a PDEIII-independent mechanism that is differentiation context-specific and does not involve inhibition of MPL-mediated early signal transduction events PMID: 20586925
  14. Data show that PDE3A inhibition augments CFTR-dependent submucosal gland secretion and actin skeleton disruption decreases secretion. PMID: 20089840
  15. There was a concentration-dependent inhibition in the increase of intracellular Ca2+ with PDE3 inhibition and significantly less with PDE2 inhibition. PMID: 12038792
  16. These findings suggest that alternative processing of the PDE3A gene results in the generation of two mRNAs and three protein isoforms in cardiac myocytes that differ in intracellular localization and may be regulated through different signaling pathways. PMID: 12154085
  17. PDE3A gene transcript levels are increased in the intrapulmonary and resistance pulmonary arteries by chronic hypoxia. PMID: 12466227
  18. Presence of phosphodiesterase type 3 A (PDE 3A) mRNA was confirmed in human germinal vesicle-stage (GV) oocytes. A selective PDE 3 inhibitor, Org 9935, arrested oocytes retrieved from immature follicles in prophase I for up to 72 h. PMID: 12773402
  19. PDE3, but not of PDE5, extends to all major processes in thrombus formation: assembly of platelets into aggregates, secretion of autocrine products, and procoagulant activity. PMID: 15041473
  20. 14-3-3 Proteins can discriminate between sites in a region of multisite phosphorylation on PDE3A; the cytoskeletal cross-linker protein plectin-1 coimmunoprecipitated with PDE3A independently of 14-3-3 binding. PMID: 16153182
  21. cGMP may amplify cAMP-mediated signaling in intracellular compartments of myocardium by PDE3-dependent and PDE3-independent mechanisms. PMID: 16172121
  22. structure-function analysis of the catalytic region of human platelet phosphodiesterase 3A PMID: 16873361
  23. The positive-feedback loop described in this review is regulated by phosphodiesterase 3A and inducible cAMP early repressor (ICER) and is pathologically important in adult hearts. PMID: 17332439
  24. activation/phosphorylation of PDE3A via Akt signaling pathway participates in regulating cAMP during thrombin activation of platelets. PMID: 17392505
  25. platelet activation stimulates PKC-dependent phosphorylation of PDE3A on Ser(312), Ser(428), Ser(438), Ser(465), and Ser(492) leading to a subsequent increase in cAMP hydrolysis and 14-3-3 binding. PMID: 19261611

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Involvement in disease Hypertension and brachydactyly syndrome (HTNB)
Subcellular Location Membrane; Multi-pass membrane protein.
Protein Families Cyclic nucleotide phosphodiesterase family, PDE3 subfamily
Database Links

HGNC: 8778

OMIM: 112410

KEGG: hsa:5139

STRING: 9606.ENSP00000351957

UniGene: Hs.386791

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