Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

TRMT112

Uniprot No.

Q9UI30

Research Area

Metabolism

Alternative Names

TRMT112; AD-001; HSPC152; HSPC170; Multifunctional methyltransferase subunit TRM112-like protein; tRNA methyltransferase 112 homolog

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-125aa

Target Protein Sequence

MKLLTHNLLSSHVRGVGSRGFPLRLQATEVRICPVEFNPNFVARMIPKVEWSAFLEAADNLRLIQVPKGPVEGYEENEEFLRTMHHLLLEVEVIEGTLQCPESGRMFPISRGIPNMLLSEEETES
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

30.2kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-125 constitute the expression domain of recombinant Human TRMT112. The expected molecular weight for the TRMT112 protein is calculated to be 30.2 kDa. Expression of this TRMT112 protein is conducted in e.coli. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of TRMT112, making it easier to detect and purify the TRMT112 recombinant protein in the later stages of expression and purification.

The human tRNA methyltransferase 112 homolog (TRMT112) is a protein involved in tRNA modification. TRMT112 is essential for the proper functioning of the tRNA modification machinery. TRMT112 associates with various tRNA methyltransferases, aiding in the methylation of specific nucleotides within tRNAs. This methylation process contributes to the structural stability and accurate translation of mRNA into proteins during protein synthesis. Research on TRMT112 extends to understanding its role in ensuring the fidelity of translation and its potential implications in various cellular processes. Exploring TRMT112 provides valuable insights into the intricate regulatory mechanisms governing protein synthesis and broader cellular functions.

Target Background

Function

Acts as an activator of both rRNA/tRNA and protein methyltransferases. Together with methyltransferase BUD23, methylates the N(7) position of a guanine in 18S rRNA. The heterodimer with HEMK2/N6AMT1 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. The heterodimer with HEMK2/N6AMT1 also monomethylates 'Lys-12' of histone H4 (H4K12me1). The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species. Involved in the pre-rRNA processing steps leading to small-subunit rRNA production. Together with methyltransferase METTL5, specifically methylates the 6th position of adenine in position 1832 of 18S rRNA.

Gene References into Functions

The localization of the TRMT112 protein is determined by WBSCR22, and the WBSCR22-TRMT112 complex is localized in the cell nucleus. PMID: 26214185

Subcellular Location

Nucleus, nucleoplasm. Cytoplasm, perinuclear region.

Protein Families

TRM112 family

Database Links

HGNC: 26940

KEGG: hsa:51504

STRING: 9606.ENSP00000438349

UniGene: Hs.333579

Code	CSB-EP883416HU
Abbreviation	Recombinant Human TRMT112 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP883416HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) TRMT112. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP883416HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) TRMT112.
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Recombinant Human tRNA methyltransferase 112 homolog (TRMT112)

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