Recombinant Human tRNA wybutosine-synthesizing protein 2 homolog (TRMT12)

Code CSB-YP687495HU
MSDS
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Source Yeast
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Code CSB-EP687495HU
MSDS
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Source E.coli
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Code CSB-EP687495HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP687495HU
MSDS
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Source Baculovirus
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Code CSB-MP687495HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TRMT12
Uniprot No.
Alternative Names
Alpha amino alpha carboxypropyl transferase TYW2; FLJ20772; Homolog of yeast tRNA methyltransferase; Radical S-adenosyl methionine and flavodoxin domain-containing protein 2; TRM 12; TRM12; TRMT 12; TRMT12; tRNA methyltransferase 12; tRNA methyltransferase 12 homolog; tRNA wybutosine-synthesizing protein 2 homolog; tRNA-yW-synthesizing protein 2; TYW2; TYW2_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-448
Target Protein Sequence
MRENVVVSNM ERESGKPVAV VAVVTEPWFT QRYREYLQRQ KLFDTQHRVE KMPDGSVALP VLGETLPEQH LQELRNRVAP GSPCMLTQLP DPVPSKRAQG CSPAQKLCLE VSRWVEGRGV KWSAELEADL PRSWQRHGNL LLLSEDCFQA KQWKNLGPEL WETVALALGV QRLAKRGRVS PDGTRTPAVT LLLGDHGWVE HVDNGIRYKF DVTQCMFSFG NITEKLRVAS LSCAGEVLVD LYAGIGYFTL PFLVHAGAAF VHACEWNPHA VVALRNNLEI NGVADRCQIH FGDNRKLKLS NIADRVILGL IPSSEEGWPI ACQVLRQDAG GILHIHQNVE SFPGKNLQAL GVSKVEKEHW LYPQQITTNQ WKNGATRDSR GKMLSPATKP EWQRWAESAE TRIATLLQQV HGKPWKTQIL HIQPVKSYAP HVDHIVLDLE CCPCPSVG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86.
Gene References into Functions
  1. analysis by RT-qPCR using RNA from 30 breast tumors showed TRMT12 was overexpressed >2 fold in 87% of the tumors. TRMT12 is a homologue of a yeast gene encoding a tRNA methyltransferase involved in the posttranscriptional modification of tRNA(Phe) PMID: 17440925
Protein Families
Class I-like SAM-binding methyltransferase superfamily, TRM5/TYW2 family
Database Links

HGNC: 26091

OMIM: 611244

KEGG: hsa:55039

STRING: 9606.ENSP00000329858

UniGene: Hs.9925

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