Recombinant Intermediate filament protein ifa-2 (ifa-2), partial

Code CSB-YP522331CXY
MSDS
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Source Yeast
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Code CSB-EP522331CXY
MSDS
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Source E.coli
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Code CSB-EP522331CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP522331CXY
MSDS
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Source Baculovirus
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Code CSB-MP522331CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mua-6
Uniprot No.
Alternative Names
mua-6; ifa-2; W10G6.3Intermediate filament protein ifa-2; Cel IF A2; Intermediate filament protein A2; IF-A2
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cytoplasmic intermediate filaments provide mechanical strength to cells. Essential protein, involved in attachment structures in epidermal cells that connect muscles to the external cuticle. Probably acts by forming hypodermal hemidesmosome complexes that help mediate muscle-cuticle force transduction. Although expressed during embryogenesis, it is not required for embryonic development of muscle-cuticle linkages nor for the localization of other proteins to the hemidesmosomes in embryos.
Gene References into Functions
  1. Results suggest that the head domain contains redundant signals for IF localization, whereas non-redundant essential functions map to the IFA-2 tail, although the tail is unlikely to be directly involved in fibrous organelle localization. PMID: 25742641
Subcellular Location
Cell junction, hemidesmosome. Note=Colocalizes with myoactin.
Protein Families
Intermediate filament family
Tissue Specificity
Mainly expressed in regions of the hypodermis adjacent to muscle. Expressed in longitudinal stripes where the mechanosensory neurons interface with the hypodermis. Also expressed to the uterine seam and within the uterine-vulval cells.
Database Links

KEGG: cel:CELE_W10G6.3

STRING: 6239.W10G6.3.2

UniGene: Cel.17660

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