Recombinant Lipoprotein mxiM (mxiM)

Code	CSB-YP358133SZB
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP358133SZB
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP358133SZB-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP358133SZB
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP358133SZB
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

mxiM

Uniprot No.

P0A1X2

Alternative Names

mxiM; sctG; CP0143; Type 3 secretion system pilotin; Lipoprotein MxiM; MxiD pilot; Secretin pilot protein

Species

Shigella flexneri

Expression Region

24-142

Target Protein Sequence

CALKSSS NSEKEWHIVP VSKDYFSIPN DLLWSFNTTN KSINVYSKCI SGKAVYSFNA GKFMGNFNVK EVDGCFMDAQ KIAIDKLFSM LKDGVVLKGN KINDTILIEK DGEVKLKLIR GI

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in the synthesis of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. Pilot protein that is required for the proper localization of the secretin MxiD/SctC in the outer membrane. Also influences both MxiD/SctC multimerization and stability. Required for both Ipa translocation and tissue culture cell invasion. Binds lipids.

Gene References into Functions

The structure of MxiM28-142, the Shigella pilot protein that is essential for the assembly and membrane association of the Shigella secretin, MxiD, was determined at 1.5 A. PMID: 15775974
The mutually exclusive association of lipids and MxiD with MxiM establishes a framework for understanding the role of a pilot in the outer-membrane insertion and multimerization of the secretin ring. PMID: 18940609

Subcellular Location

Cell outer membrane; Lipid-anchor; Periplasmic side.

Database Links

KEGG: sfl:CP0143

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