Recombinant Listeria monocytogenes serovar 1/2a Listeriolysin O (hly), partial

Code CSB-YP320184LPY
MSDS
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Source Yeast
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Code CSB-EP320184LPY
MSDS
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Source E.coli
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Code CSB-EP320184LPY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP320184LPY
MSDS
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Source Baculovirus
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Code CSB-MP320184LPY
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
hly
Uniprot No.
Alternative Names
hly; hlyA; lisA; lmo0202; Listeriolysin O; LLO; Thiol-activated cytolysin
Species
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
A cholesterol-dependent pore-forming toxin, which is a major virulence factor required for the escape of bacteria from phagosomal vacuoles and entry into the host cytosol. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Listeriolysin O activates mitogen-activated protein (MAP) kinase activity in host cells, most likely as a result of the permeabilization of the host cell membrane. Also induces a proteasome-independent degradation of UBE2I (the SUMO-conjugating enzyme UBC9) and a proteasome-dependent degradation of some sumoylated proteins. Finally, is necessary and sufficient for spacious Listeria-containing phagosomes (SLAPs) formation, suggesting a role for listeriolysin O in promoting L.monocytogenes replication in vacuoles, leading to persistent infection. Recognized by serum from healthy humans exposed to L.monocytogenes as well from patients who have recovered from listeriosis.
Gene References into Functions
  1. Listeriolysin O -induced down-regulation of Ubc9 is independent of Ubc9-SUMO interaction, however, it may involve phosphorylation signaling. PMID: 28911869
  2. During infection, PML undergoes oxidation-mediated multimerization, associates with the nuclear matrix, and becomes de-SUMOylated due to the pore-forming activity of the Listeria toxin listeriolysin O (LLO). PMID: 28074026
  3. The current MD simulation work provides insights into the mechanism by which the compounds inhibit LLO at the atomic level and will be useful for the development of new, selective LLO inhibitors. PMID: 25749652
  4. Listeria L. monocytogenes induced decrease in hTERT levels is triggered by listeriolysin O (LLO). PMID: 23006849
  5. findings show that listeriolysin O and other cholesterol-dependent cytolysins induce K+ efflux through pore formation at the cell membrane PMID: 21482680
  6. Data suggest that CFTR transiently increases phagosomal chloride concentration after infection, potentiating LLO pore formation and vacuole lysis. PMID: 21220348
  7. These data showed that listeriolysin O-mediated cytoplasmic entry of Listeria monocytogenes could induce the activation of intracellular calcium signaling, which is essential for maturation and secretion of IL-1 alpha in macrophages. PMID: 20194588
  8. L. monocytogenes-infected human PBMCs produced IL-1beta, largely depending on an LLO-mediated phagosomal rupture and cathepsin B release, which is sensed by Nlrp3 PMID: 20008285
  9. These results reveal the importance of the 91-99 region in the production of a stable and functional LLO. LD(50) determinations in the mouse model suggest a possible link between LLO stability and virulence. PMID: 16622046
  10. Listeria monocytogenes-induced caspase-1 activation and interferon-gamma production in mouse macrophages is ascribed mainly to the presence of listeriolysin O. PMID: 18523249

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Subcellular Location
Secreted. Host membrane; Multi-pass membrane protein. Host cell membrane; Multi-pass membrane protein.
Protein Families
Thiol-activated cytolysin family
Database Links

KEGG: lmo:lmo0202

STRING: 169963.lmo0202

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