Recombinant Macaca mulatta (Rhesus macaque) Prostate-specific antigen (KLK3)

The target gene KLK3 (PSA) was constructed into an expression vector, transformed into E.coli Rosetta2(DE3) and expressed by IPTG, and the protein size was detected by SDS-PAGE. Purifying by nickel column affinity chromatography to obtain the target protein.
Taking EP system as an example, the general steps of purification are as follows: firstly, balance the column with buffer solution, and ultrasonically break the bacterial solution, then take the supernatant, filter and load the sample. secondly elute with eluents with different gradients, collect the corresponding elution peaks and detect the target bands.
DTT or mercaptoethanol will be added when denatured SDS-PAGE is used to detect the protein, but this is only to detect the size of the protein, and there is no DTT or TCEP in the protein preparation process and protein buffer.
Please remark when placing an order: DTT or TCEP is not included in the preparation process and protein buffer.