Recombinant Meiotic spindle formation protein mei-1 (mei-1)

Code CSB-YP330397CXY
MSDS
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Source Yeast
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Code CSB-EP330397CXY
MSDS
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Source E.coli
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Code CSB-EP330397CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP330397CXY
MSDS
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Source Baculovirus
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Code CSB-MP330397CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mei-1
Uniprot No.
Alternative Names
mei-1; T01G9.5Meiotic spindle formation protein mei-1; EC 5.6.1.1; Katanin p60 ATPase-containing subunit A1; Katanin p60 subunit A1; p60 katanin
Species
Caenorhabditis elegans
Expression Region
1-472
Target Protein Sequence
MNGDVQSVIR GYLERAQVAK TMSDAGRWNE AGDLLRQLMT DVKSCKISAS NRDEHDARNT FLRALEANLK LVQQNVRDED DLHEAMTRQS GSPEPPADPD VWSKPSPPLP SSSKFGATKK GVGAAGPRPR EISKSTSSMS TNPADVKPAN PTQGILPQNS AGDSFDASAY DAYIVQAVRG TMATNTENTM SLDDIIGMHD VKQVLHEAVT LPLLVPEFFQ GLRSPWKAMV LAGPPGTGKT LIARAIASES SSTFFTVSST DLSSKWRGDS EKIVRLLFEL ARFYAPSIIF IDEIDTLGGQ RGNSGEHEAS RRVKSEFLVQ MDGSQNKFDS RRVFVLAATN IPWELDEALR RRFEKRIFIP LPDIDARKKL IEKSMEGTPK SDEINYDDLA ARTEGFSGAD VVSLCRTAAI NVLRRYDTKS LRGGELTAAM ESLKAELVRN IDFEAALQAV SPSAGPDTML KCKEWCDSFG AM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays. Required specifically for meiotic spindle formation in the female germline; the presence of this protein is inimical to the formation of mitotic spindles. In body wall muscles, regulates organization of myosin thick filaments.
Gene References into Functions
  1. The reconstructions show that katanin cycles between open spiral and closed ring conformations, depending on the ATP occupancy of a gating protomer that tenses or relaxes interprotomer interfaces. PMID: 28783150
  2. mei-1 and aspm-1 promote oocyte meiotic spindle pole assembly PMID: 24554763
  3. The phosphorylation of MEI-1 may ensure temporal activation of the katanin complex during meiosis, whereas CRL3(MEL-26)-mediated degradation of both MEI-1 and its activator PPFR-1 ensure efficient katanin inactivation in the transition to mitosis. PMID: 23918937
  4. MEI-1 is required for translocation of the meiosis I spindle to the oocyte cortex. PMID: 12885567
  5. The BTB-containing protein MEL-26 is a component required for degradation of MEI-1 in vivo; importantly, MEL-26 specifically interacts with CUL-3 and MEI-1 in vivo and in vitro, and displays properties of a substrate-specific adaptor PMID: 13679921
  6. the identification of BTB-domain protein MEL-26 and its genetic target MEI-1 as substrate-specific adaptors for C. elegans CUL-3 PMID: 13679922
  7. MEI-1 is the only essential target for MEL-26, and possibly for the E3 ubiquitin ligase CUL-3, but the upstream ubiquitin ligase activating enzyme RFL-1 has additional essential targets PMID: 19361490

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Subcellular Location
Cytoplasm, cytoskeleton, spindle pole. Chromosome. Cytoplasm.
Protein Families
AAA ATPase family, Katanin p60 subunit A1 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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