Recombinant Methyltransferase-like protein 16 homolog (mett-10)

Code CSB-YP600995CXY
MSDS
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Source Yeast
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Code CSB-EP600995CXY
MSDS
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Source E.coli
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Code CSB-EP600995CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP600995CXY
MSDS
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Source Baculovirus
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Code CSB-MP600995CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mett-10
Uniprot No.
Alternative Names
mett-10; ZK1128.2U6 small nuclear RNA; adenine-(43)-N(6))-methyltransferase; EC 2.1.1.346
Species
Caenorhabditis elegans
Expression Region
1-479
Target Protein Sequence
MSQNNEMHPR NPYRNKPPDF KALAVEYPEF RKFCQYVSNG KVTFDFKKDA AVRCLTQTLL KKDFNLDVEI PPGHLVPRVP QKLNYCLLID DLLKANKLTK NVIGIDIGTG TSCIHALIGA RQFNWKFIAT DGDEKSVRVA HENVAKNGLS SSICVVHVNP DVKTVLMDVV NTIPDTDYAF CMCNPPFFEK GNGDDKFCED ISSSTETYSN RVASEFRTAP HSATFASSAE LFVDGGEVAF VNRIIDDSVL LRDRIKIYTT MIGRKSSLKP LQNRLQRFGD DVKIMISVLN QGKTKRWMLA WTFSKSVSLT TIDRPISFQC PKPGLTRLMQ EISILNGRLR QEDTLAIVAE FKCVTWTNQR ARKRAKAILS ESSIKKAKWN FSNVACQVAF GAGDGKDSYT DAGNFVSSES IPTNNLNAWD NASQAYFPLP NGEVPGPIIR IRIQVFSEDS YDSISFELIS GSKQHLHQLV QYLKNLICR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating splicing of S-adenosylmethionine synthase transcripts (sams-3, sams-4 and sams-5). Able to N6-methylate a subset of mRNAs containing the 5'UACAGAAAC-3' nonamer sequence. Plays a key role in S-adenosyl-L-methionine homeostasis: under rich-diet conditions, catalyzes N6-methylation of S-adenosylmethionine synthase mRNAs (sams-3, sams-4 and sams-5), directly inhibiting splicing and protein production of S-adenosylmethionine synthase. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA). Required for gamete production, inhibiting germ cell proliferative fate and ensuring germ cell meiotic development. Also promotes progression of the mitotic cell cycle in those germ cells that continue to proliferate. Plays a role in the development of the vulva, somatic gonad and embryo.
Gene References into Functions
  1. METT-10 functions to inhibit germ-cell proliferative fate, despite promoting mitotic cell cycle progression of those germ cells that do proliferate, separates the specification of proliferative fate from its execution. PMID: 19596901
Subcellular Location
Nucleus.
Protein Families
Methyltransferase superfamily, METTL16/RlmF family
Tissue Specificity
Expressed in the intestine, vulva, and cells of the somatic gonad including distal tip cells, gonadal sheath cells and spermatheca.
Database Links

KEGG: cel:CELE_ZK1128.2

STRING: 6239.ZK1128.2a

UniGene: Cel.9495

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