Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Plcd1

Uniprot No.

Q8R3B1

Research Area

Signal Transduction

Species

Mus musculus (Mouse)

Source

Baculovirus

Expression Region

1-756aa

Target Protein Sequence

MDSGRDFLTLHGLQDDPDLQALLKGSQLLKVKSSSWRRERFYKLQEDCKTIWQESRKVMRSPESQLFSIEDIQEVRMGHRTEGLEKFARDIPEDRCFSIVFKDQRNTLDLIAPSPADVQHWVQGLRKIIDRSGSMDQRQKLQHWIHSCLRKADKNKDNKMNFKEVKDFLKELNVQVDDSYARKIFRECDHSQTDSLEDEEIETFYRMLTQRAEIDRAFAEAAGSAETLSVEKLVTFLQHQQREEEAGPALALSLIERYEPSETAKAQRQMTKDGFLMYLLSADGNAFSLAHRRVYQDMNQPLSHYLVSSSHNTYLLEDQLTGPSSTEAYIRALCKGCRCLELDCWDGPNQEPIIYHGYTFTSKILFCDVLRAIRDYAFKASPYPVILSLENHCSLEQQRVMAHHLRAILGPMLLDQPLDGVTTSLPSPEQLKEKILLKGKKLGGLLPAGGENGPEATDVSDEDEAAEMEDEAVRSQVQHKPKEDKLKLVPELSDMVIYCKSVHFGGFSSPSTSGQAFYEMASFSESRALRLLQESGNSFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYLGCFQDNGGCGYVLKPAFLRDPDTTFNSRALTQGPWWAPKKLRVWIISGQQLPKVNKNKNSIVDPKVIVEIHGVGQDVASRQTAVITNNGFNPRWDTEFEFVVAVPDLALVRFMVEDYDSSSKNDFIGQSTIPWNSLKQGYRHVHLLSKNGDLHPSATLFVKISIQD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

91.5 kDa

Protein Length

Full Length

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant mouse 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (Plcd1) is produced using a baculovirus expression system. The transfer plasmid contains the full-length mouse Plcd1 protein, spanning amino acids 1 to 756, along with a C-terminal 6xHis-tag for purification. Insect cells are co-transfected with a mixture of the transfer plasmid and baculovirus DNA construct, facilitating the generation of the recombinant baculovirus particles. After that, infecting insect cells with the recombinant baculovirus carrying the Plcd1 expression construct. The infected cells then produce and accumulate the recombinant Plcd1 protein. The purified recombinant Plcd1 protein exhibits a purity of up to 85%, as determined by SDS-PAGE analysis. On the gel, the Plcd1 protein migrates as a band with an approximate molecular weight of 92 kDa.

Plcd1 is involved in various cellular processes, including intracellular calcium signaling, regulation of ion channels, and modulation of the protein kinase C (PKC) signaling pathway. By regulating the levels of IP3 and DAG, Plcd1 contributes to the dynamic control of cellular responses, such as gene expression, cytoskeletal rearrangement, and cell proliferation.

Target Background

Function

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development. Binds phosphatidylinositol 4,5-bisphosphate.

Gene References into Functions

Data show that phospholipase C delta1 (PLCD1) transfection can induce the apoptosis of CAPAN-1 and BXPC-3 pancreatic cancer cells, arrest the cell cycle in G0 /G1 phase. PMID: 27371838
the Ca(2+) dependence of the interaction between PRIP-C2 and Syt1-C2A was attributed to Ca(2+) binding with Syt1-C2A, but not PRIP-C2, using a series of mutants prepared from both C2 domains. PMID: 25242442
Results indicate that PLCdelta1 and PLCdelta3 are required for cardiomyocyte survival and normal cardiac function. PMID: 24810051
Expression of PLCdelta1 is increased in ALS mouse spinal cord and in neurons from ALS mice. Genetic ablation of this protein in ALS mice significantly increases survival PMID: 23969236
Identification, characterization and regulatory properties of a novel PLCD1 isoform in mouse. PMID: 23892088
Plcd1 and Plcd3 have synergistic effects on the murine hair follicle in specific regions of the body surface. PMID: 22723964
Loss of phospholipase Cdelta1 from keratinocytes causes features of interleukin-17-associated inflammatory skin diseases. PMID: 22805570
PLCdelta1 has essential roles in thermogenesis and adipogenesis and thereby contributes to the development of obesity. PMID: 21617180
Results suggest that phospholipase C delta(1) is required for skin stem cell lineage commitment. PMID: 12805213
the PLC-delta1 promoter is under the control of NF-kappaB, which mediates the expression of PLC-delta due to Abeta42 PMID: 14550290
PLCdelta(1) and PLCdelta(4) are probably differentially regulated in distinct cellular compartments by PI(4,5)P(2) PMID: 15037625
Nuclear translocation of phospholipase C-delta1 is linked to the cell cycle and nuclear phosphatidylinositol 4,5-bisphosphate PMID: 15809301
Data indicate that receptor-triggered phospholipase C activity is enhanced by positive feedback from Ca2+ entering the cytoplasm from intracellular stores and via store-operated channels in the plasma membrane. PMID: 16159958
PLCdelta1 and PLCdelta3 are essential in trophoblasts for placental development. PMID: 16314520
Taken together, these results indicate that lack of PLC-delta1 induces skin inflammation and that the epidermal hyperplasia in PLC-delta1 KO mice is caused by skin inflammation. PMID: 17397799
These results indicate that PLC-delta1 is an essential molecule downstream of Foxn1 in normal hair formation, and strongly suggest that hairlessness in nude mice is caused by insufficient expression of PLC-delta1. PMID: 17938256
PLCd1 is essential for normal hair formation, and PLCd1 KO mice displayed symptoms of skin inflammation. PLCdelta 1 KO mice possess skin phenotype similar to nude mice. PMID: 18177742

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Tissue Specificity

Highly expressed in brain, heart, lung, epididymis and testis. Detected at lower levels in kidney and skeletal muscle.

Database Links

KEGG: mmu:18799

STRING: 10090.ENSMUSP00000010804

UniGene: Mm.23963

Code	CSB-BP854509MO
Abbreviation	Recombinant Mouse Plcd1 protein
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (Plcd1)

Product Details

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