Product Details

Purity

>85% (SDS-PAGE)

Target Names

Arl13b

Uniprot No.

Q640N2

Alternative Names

Arl13b; Arl2l1ADP-ribosylation factor-like protein 13B; ADP-ribosylation factor-like protein 2-like 1; ARL2-like protein 1; Protein hennin

Species

Mus musculus (Mouse)

Expression Region

1-427

Target Protein Sequence

MFSLMANCCN LFKRWREPVR KVTLVMVGLD NAGKTATAKG IQGEHPEDVA PTVGFSKIDL RQGKFQVTIF DLGGGKRIRG IWKNYYAESY GVIFVVDSSD EERMEETKET MSEVLRHPRI SGKPILVLAN KQDKEGALGE ADVIECLSLE KLVNEHKCLC QIEPCSAVLG YGKKIDKSIK KGLYWLLHII AKDFDALSER IQKDTTEQRA LEEQEKRERA ERVRKLREER EREQTELDGT SGLAEIDSGP VLANPFQPIA AVIIENEKKQ EKEKKKQTVE KDSDVGLLEH KVEPEQAAPQ SEADCCLQNP DERVVDSYRE ALSQQLDSED EQDQRGSESG ENSKKKTKKL RMKRSHRVEP VNTDESTPKS PTPPQPPPPV GWGTPKVTRL PKLEPLGETR HNDFYGKPLP PLAVRQRPNG DAQDTIS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Cilium-specific protein required to control the microtubule-based, ciliary axoneme structure. May act by maintaining the association between IFT subcomplexes A and B. Binds GTP but is not able to hydrolyze it; the GTPase activity remains unclear. Required to pattern the neural tube. Involved in cerebral cortex development: required for the initial formation of a polarized radial glial scaffold, the first step in the construction of the cerebral cortex, by regulating ciliary signaling. Regulates the migration and placement of postmitotic interneurons in the developing cerebral cortex.

Gene References into Functions

High Arl13b expression is associated with medulloblastoma formation. PMID: 29378965
Arl13b dysfunction resulted in hennin cell stagnation due to poor growth factor signaling and impaired detection of extracellular electrical gradients. PMID: 26963749
Biochemical characterization of purified mammalian ARL13B protein indicates that it is an atypical GTPase and ARL3 guanine nucleotide exchange factor (GEF).( PMID: 28487361
To investigate whether Arl13b has a role in ciliogenesis in mammalian kidney and whether loss of function of Arl13b leads to cystic kidneys in mammals, we generated a mouse model with kidney-specific conditional knockout of Arl13b Deletion of Arl13b in the distal nephron at the perinatal stage led to a cilia biogenesis defect and rapid kidney cyst formation PMID: 27153923
The authors show that the ciliary G-protein Arl13B, mutated in Joubert syndrome, is the guanine nucleotide exchange factor for Arl3, and its function is conserved in evolution PMID: 26551564
A new role for Arl13b in actin cytoskeleton remodeling through the interaction with Myh9. PMID: 24777479
Early neuroepithelial deletion of ciliary Arl13b reversed the apical-basal polarity of radial progenitors & caused aberrant neuronal placement. Arl13b signaling in primary cilia is crucial for initial formation of a polarized radial glial scaffold. PMID: 23817546
guidance cue receptors essential for interneuronal migration localize to interneuronal primary cilia, but their concentration and dynamics are altered in the absence of Arl13b PMID: 23153492
By deleting Arl13b in mouse, we induced low-level constitutive GliA function at specific developmental stages and defined a crucial period prior to E10.5 when shifts in the level of GliA cause cells to change their fate PMID: 23014696
Study shows the cilia protein Arl13b is required for left right axis specification as its absence results in heterotaxia; the defect originates in the node where Cerl2 is not downregulated and asymmetric expression of Nodal is not maintained resulting in symmetric expression of both genes. PMID: 22554696
The role of Arl13b in ciliogenesis and it's interaction with Smo and Shh proteins.Arl13b regulates the ciliary entry of Smo. PMID: 21976698
Arl13bhnn mutants have abnormal ventral neural tube patterning due to disrupted sonic hedgehog signaling; in addition, dorsal patterning defects occur from abnormal bone morphogenetic protein signaling. PMID: 21539826

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Subcellular Location

Cell projection, cilium membrane; Lipid-anchor. Cell projection, cilium. Note=Associates to the cilium membrane via palmitoylation. Localizes to proximal ciliary membranes, to an inversin-like subciliary membrane compartment, excluding the transition zone.

Protein Families

Small GTPase superfamily, Arf family

Tissue Specificity

Cilium-specific protein. Expressed in neuroepithelial cells and developing radial glia of the developing cerebral cortex: present in both neuroepithelial and radial progenitor cells. In radial progenitors, found in the apical, cell soma domains of these c

Database Links

KEGG: mmu:68146

STRING: 10090.ENSMUSP00000086703

UniGene: Mm.96833

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Recombinant Mouse ADP-ribosylation factor-like protein 13B (Arl13b)

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