Recombinant Mouse AT-rich interactive domain-containing protein 5B (Arid5b), partial

Code CSB-YP805924MO
MSDS
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Source Yeast
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Code CSB-EP805924MO
MSDS
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Source E.coli
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Code CSB-EP805924MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP805924MO
MSDS
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Source Baculovirus
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Code CSB-MP805924MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Arid5b; Desrt; Mrf2AT-rich interactive domain-containing protein 5B; ARID domain-containing protein 5B; Developmentally and sexually retarded with transient immune abnormalities protein; Desrt; MRF1-like; Modulator recognition factor protein 2; MRF-2
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription coactivator that binds to the 5'-AATA[CT]-3' core sequence and plays a key role in adipogenesis and liver development. Acts by forming a complex with phosphorylated PHF2, which mediates demethylation at Lys-337, leading to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. Required for adipogenesis: regulates triglyceride metabolism in adipocytes by regulating expression of adipogenic genes. Overexpression leads to induction of smooth muscle marker genes, suggesting that it may also act as a regulator of smooth muscle cell differentiation and proliferation.
Gene References into Functions
  1. Arid5b KO reduces prostaglandin I2 signaling in primary skeletal muscle cells and attenuates myogenesis. PMID: 29196500
  2. These results strongly suggest that p62 plays a crucial role in preventing fenofibrate-induced cell death. PMID: 26282199
  3. Arid5b recruits Phf2 to the promoter region of Sox9 target genes and stimulates H3K9me2 demethylation of these genes. PMID: 24276541
  4. knockdown of Mrf-2 increases the rate of fatty acid recycling in 3T3-L1-derived adipocytes. PMID: 19913508
  5. phenotypes suggest that Mrf-2 is essential for accumulation of lipid stores in postnatal life PMID: 14651970
  6. Data suggest that Mrf-2 facilitates the induction of key adipogenic transcription factors C/EBPalpha and peroxisome proliferator-activated receptor-gamma. PMID: 17962384
  7. These results indicate that Mrf2 can be a potential regulator of adipocyte differentiation and a potential repressor of leptin. PMID: 18070594

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Subcellular Location
Nucleus.
Protein Families
ARID5B family
Tissue Specificity
Widely expressed. Expressed in lung, heart, small intestine, kidney, muscle and brain. Also expressed in spleen, thymus, endocrine organs and in uterus and testis.
Database Links

KEGG: mmu:71371

UniGene: Mm.440357

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