Recombinant Mouse Actin, aortic smooth muscle(Acta2)

Code CSB-YP001206MO
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Source Yeast
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Code CSB-EP001206MO
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Source E.coli
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Code CSB-EP001206MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001206MO
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Source Baculovirus
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Code CSB-MP001206MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Acta2
Uniprot No. P62737
Alternative Names Acta2; Actsa; ActvsActin; aortic smooth muscle; Alpha-actin-2) [Cleaved into: Actin; aortic smooth muscle; intermediate form]
Species Mus musculus (Mouse)
Expression Region 3-377
Target Protein Sequence EEEDSTAL VCDNGSGLCK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH SFYNELRVAP EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV LSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS LSTFQQMWIS KQEYDEAGPS IVHRKCF
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Gene References into Functions
  1. In murine myocardial infarction, infiltration of the infarct border zone with abundant a-SMA-positive myofibroblasts was associated with scar contraction. Isolated cardiac fibroblasts cultured in plates showed high a-SMA expression localized in stress fibers, exhibited activation of focal adhesion kinase (FAK), and synthesized large amounts of extracellular matrix proteins. PMID: 27825850
  2. These data suggest that the interplay between cell-matrix adhesion and intercellular adhesion is an important determinant for some aspects of TGFbeta1-induced epithelial-mesenchymal transition via alphaSMA expression induction. PMID: 27194451
  3. The level of alpha-SMA expression by intramuscular fibrogenic cells does not correlate positively with the level of collagen gene expression or the severity of skeletal muscle fibrosis in the mdx5cv mice. alpha-SMA is not a functional marker of fibrogenic cells in skeletal muscle fibrosis associated with muscular dystrophy PMID: 29320561
  4. Data suggest that membrane-proximal signaling complexes constrained by AKAP220 impact the actin barrier dynamics and AQP2 trafficking to ensure water homeostasis. PMID: 27402760
  5. Gremlin1 accelerates hepatic stellate cell activation through upregulation of TGF-B1, alpha-SMA, and COL1a1 expression in a liver fibrosis disease model. PMID: 28467108
  6. Disruption of smooth muscle Acta2 increases reactive oxygen species levels, activates NF-kappaB signaling and increases AngII receptor type I a expression. PMID: 28461455
  7. The expression levels of alpha-smooth muscle actin (alpha-SMA, a marker of fibroblast activation) in fibrotic livers were significantly higher than those in control livers. PMID: 27538444
  8. this study identifies Rac1 as a downstream target for SMA to inhibit smooth muscle cell proliferation and migration. PMID: 27176050
  9. the coordinated up-regulations of desmin and alpha- actinin specifically in the early stage of diastolic heart failure mouse models indicate a novel myocardial response. PMID: 26529187
  10. site-directed mutagenesis revealed several basic amino acid residues in the intermolecular (R267) and intramolecular (K82 and R159) subdomains that are essential for Purbeta transcriptional repressor function in Acta2 promoter-reporter assays. In keeping with their diminished Acta2 repressor activity in fibroblasts, purified Purbeta variants containing an R267A mutation exhibited reduced binding affinity for purine-ric... PMID: 27064749
  11. Alpha-smooth muscle actin protein and mRNA are enhanced from brain blood vessels in an Alzheimer's disease animal model. PMID: 27463512
  12. Astragaloside can delay the renal fibrosis process in diabetic mice by influencing the TGF-beta/SMADS signaling pathway and down-regulating TGF-beta1, SMAD2/3, and alpha-SMA expression. PMID: 26261569
  13. The increased expressions of the cartilage-associated proteins Collagen2 and alpha-SMA during scleral chondrogenesis accompany myopia development. PMID: 25684979
  14. Results suggest that the atypical motif asparaging-cysteine-systeine Asn(17)-Cys(18)-Cys(19) is crucial for the normal surface trafficking and function of hydroxycarboxylic acid receptor 2 protein hGPR109A. PMID: 25690651
  15. Results suggest that the atypical motif asparaging-cysteine-systeine Asn(17)-Cys(18)-Cys(19) is crucial for the normal surface trafficking and function of hydroxycarboxylic acid receptor 2 protein hGPR109A. PMID: 25690654
  16. The results suggest that the expression level of TGF-b1 and a-SMA in stromal fibroblasts may have prognostic significance in patients with clinical stage I-IIIA NSCLC after curative resection PMID: 24711139
  17. alphaSMA is a useful marker for further understanding the molecular and cellular characteristics of non-hematopoietic cells in the lymph nodes. PMID: 24845380
  18. This in vitro expression study revealed the additional profibrotic role of IL-6 in fibroblast to myofibroblast transition and in alpha smooth muscle actin induction. PMID: 23613464
  19. Data indicate that NRG-1beta up-regulated the gene expressions of Nkx2.5, GATA4, alpha-actin, MLC-2v, and ANF in a time-dependent regulation of ErbB/ERK1/2 pathways. PMID: 23606057
  20. these studies identify a pathway that culminates in the post-transcriptional regulation of utrophin A through increases in mRNA stability. PMID: 23591991
  21. sustained Akt1-induced alphaSMA synthesis markedly decreased upon RNA silencing of SRF and myocardin. PMID: 24106278
  22. This structural arrangement suggests that the cooperative assembly of the dimeric Purbeta repressor on the sense strand of the ACTA2 enhancer is dictated by the association of each subdomain with distinct purine-rich binding sites within the enhancer. PMID: 23724822
  23. Smooth muscle alpha-actin is not necessary for myofibroblast formation and function and for wound closure, and smooth muscle gamma-actin and skeletal muscle alpha-actin may be able to functionally compensate for the lack of smooth muscle alpha-actin in myofibroblasts PMID: 23253249
  24. It was found that binding between Porphyromonas gingivalis fimbriae and integrin alpha5beta1 on osteoblasts, and subsequent peripheral condensation of actin, are essential for entry of Porphyromonas gingivalis into osteoblasts. PMID: 23305098
  25. Gefitinib offers protection against lung fibrosis induced by bleomycin in mice probably by inhibiting the downstream signals of EGFR and by downregulating the expression of alpha-SMA. PMID: 21177177
  26. Monocytes and macrophages that express alpha actin preserve primitive hematopoietic cells in the bone marrow. PMID: 22983360
  27. Scx and Id1 are involved in the BMP4-Smad1-SMA signal transduction pathway besides the TGFbeta1-Smad1-SMA signaling pathway and modulate phenotypic changes in MCs in diabetic nephropathy. PMID: 22474292
  28. Data show that Acta2 deficient mice have a remarkable mammary phenotype such that dams lacking Acta2 are unable to nurse their offspring effectively. PMID: 22123032
  29. expression in mammary myoepithelial cells has the functional consequence of enhancing contractile force generation required for milk ejection PMID: 21368298
  30. Undifferentiating pericytes do not express alphaSMA. PMID: 21515289
  31. Over expression of alpha-SMA in asthmatic lung tissue is closely related to the onset of asthma. GABAAR antagonist bicuculline could inhibit the expression of alpha-SMA. PMID: 20848783
  32. alpha-SMA may be used as an effective indicator for the mid- and late stage incised wound of mice skin. PMID: 20225612
  33. Podocyte BK(Ca) channels are regulated by synaptopodin, Rho, and actin microfilaments. PMID: 20630939
  34. TGF-beta(1) increased the expression of alpha-SMA in BALB/c 3T3 fibroblasts and affected the Egr-1 protein expression during the early course of fibroblasts differentiation into myofibroblasts. PMID: 17651609
  35. vascular smooth muscle alpha-actin gene transcription is regulated by purbeta PMID: 12874279
  36. smooth muscle alpha-actin expression requires a transforming growth factor-beta control element PMID: 12970361
  37. Interplay between Pur repressor isoforms and Sp1 and Smad coactivators may regulate alpha-actin enhancer output in TGFbeta1-activated myofibroblasts during episodes of wound repair and tissue remodeling. PMID: 15282343
  38. The level of lung fibroblast alpha-smooth muscle actin (SMA) correlates with level of CCAAT/enhancer-binding protein beta isoform expression; SMA expression levels progressively decrease in cells from C/EBP beta wild-type to homozygous knockout mice. PMID: 15383601
  39. Pur alpha and Pur beta repress smooth muscle alpha-actin gene transcription by means of DNA strand-selective cis-element binding and cell type-dependent protein-protein interactions PMID: 16436378
  40. Smooth muscle alpha-actin suppresses the pro-fibrotic properties of myofibroblasts, highlighting the significance of smooth muscle-related proteins in moderating chronic organ fibrosis under pathological conditions. PMID: 17090535
  41. In connective tissue fibroblasts, alpha-SMA is located within nuclear invaginations in close proximity to the nuclear membrane, but not in the nucleoplasm. PMID: 17310383
  42. although both smooth muscle cells and myofibroblasts express smooth muscle alpha-actin, they use distinct transcriptional control mechanisms for regulating its expression PMID: 17823374
  43. Activation of the normally silent SM alpha A gene in reprogrammed adult cardiomyocytes is linked to elevated interaction of serum response factor with Pur-alpha/Pur-beta. PMID: 18344281
  44. Barx2 cooperates with other muscle-expressed transcription factors to regulate the early cytoskeletal remodeling events that underlie efficient myoblast differentiation. PMID: 19269978
  45. These findings indicate the existence of alpha-smooth muscle actin-positive endothelial cells in adult aortic endothelium and the possible association with progression of atherosclerosis. PMID: 19285011
  46. Study uncovers a strong reciprocal interdependence between Nck and PI(4,5)P(2) in promoting localized N-WASp-mediated actin polymerization in cells. PMID: 19917259

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Subcellular Location Cytoplasm, cytoskeleton.
Protein Families Actin family
Database Links

KEGG: mmu:11475

STRING: 10090.ENSMUSP00000048218

UniGene: Mm.213025

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