Recombinant Mouse Afadin (Mllt4), partial

Code CSB-YP865614MO
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Source Yeast
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Code CSB-EP865614MO
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Source E.coli
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Code CSB-EP865614MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865614MO
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Source Baculovirus
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Code CSB-MP865614MO
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Source Mammalian cell
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Product Details

>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Afdn; Af6; Mllt4Afadin; Afadin adherens junction formation factor; Protein Af-6
Mus musculus (Mouse)
Protein Length
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm. Essential for the organization of adherens junctions.
Gene References into Functions
  1. afadin is a critical molecule for cardiac protection against chronic pressure overload PMID: 28045017
  2. Loss of AFDN impaired the formation of adherens junctions and tight junctions. PMID: 29431241
  3. In the 2 pressure overload models, myocardial afadin is involved in mechanical stress-induced, but not pharmacological Ang II-related, compensated cardiac hypertrophy. PMID: 28659552
  4. These results indicate that afadin plays multiple roles in the complex ultrastructural morphogenesis of hippocampal mossy fiber synapses. PMID: 28498492
  5. Findings indicate that afadin plays an essential role in regulating apical-basal polarity and adherens junction integrity of radial glial cells. PMID: 28482867
  6. Codepletion of the actomyosin regulator RhoA and Afadin results in defects in the central lumens and arrests lumen remodeling PMID: 29330353
  7. Glutamate release and the postsynaptic responsiveness to glutamate were markedly reduced, but not completely lost, in the afadin-deficient mossy fiber synapse, whereas neither long-term potentiation nor long-term depression was affected. PMID: 28631873
  8. Afadin regulates the differentiation of the CA3 pyramidal cells, elongation of the mossy fiber trajectory and the density of mossy fiber synapses. PMID: 28041940
  9. afadin determines lumen placement by directing apical-basal spindle orientation, resulting in a continuous lumen and normal tubule morphogenesis. PMID: 28860115
  10. Results indicate that afadin is required for the maintenance of the radial glial scaffold for neuronal migration and that the genetic ablation of afadin leads to the formation of double cortex PMID: 25988834
  11. Here, the first crystal structure of the AFPDZ in complex with the nectin-3 C-terminal peptide containing the class II motif is reported. PMID: 25534554
  12. Afadin plays a role in the restricted localization of Paneth cells at the base of the crypt by maintaining their adhesion to adjacent crypt cells and inhibiting their movement toward the top of villi. PMID: 25333284
  13. S-afadin-specific C-terminal inserts may be involved in its preference of binding to nectin-3 and raise the possibility that there are proteins other than nectins that more preferentially bind s-afadin than l-afadin. PMID: 25263091
  14. A remarkable function of afadin was revealed, it was able to enhance cytokine expression through Rap1 activation in keratinocytes during inflammation. PMID: 25297509
  15. Afadin regulates puncta adherentia junction formation and presynaptic differentiation in hippocampal neurons. PMID: 24587018
  16. This study showed that the adherens junction proteins afadin and CDH2 are critical for the control of cell proliferation in the dorsal telencephalon and for the formation of its normal laminar structure. Inactivation. PMID: 25100583
  17. Genetic deletion of afadin causes hydrocephalus by destruction of adherens junctions in radial glial and ependymal cells in the midbrain. PMID: 24236178
  18. Results indicate that PLEKHA7 plays a cooperative role with nectin and afadin in the proper formation of Adherens junction (AJ) in epithelial cell. PMID: 23990464
  19. Afadin acts upstream of the Par complex to regulate the integration and/or coalescence of membrane microdomains, establishing apical-basal polarity and lumen formation/elongation during kidney tubulogenesis. PMID: 23487309
  20. Leukemic transformation by the MLL-AF6 fusion oncogene requires the H3K79 methyltransferase Dot1l. PMID: 23361907
  21. study identifies afadin as an effector of R-Ras in cortical neurons and demonstrate a novel signaling pathway involved in regulation of axon branching PMID: 22593211
  22. both AF6 and MUPP1 are associated with Cx36-containing interneuronal gap junctions that form electrical synapses in adult rodent brain PMID: 22211808
  23. The results indicate that ADIP plays an essential role in PDGF-induced cell movement by interacting with afadin and Vav2 and regulating the activation of Rac. PMID: 22027834
  24. Afadin is a key intracellular signaling molecule for cadherin recruitment and is necessary for spine and synapse formation in vivo. PMID: 22219273
  25. findings suggest that afadin plays crucial roles, independently of the role as the nectin-afadin module, in barrier function and homeostasis of the intestinal epithelia once the epithelial structure has been established PMID: 21652626
  26. the Necl-5-nectin, nectin-nectin, and nectin-afadin interactions cooperatively increase the clustering of the nectin-afadin complex at the cell-cell contact sites, promoting the formation of the nectin-based cell-cell adhesion. PMID: 21880730
  27. AF6/afadin is a marker of poor outcome in breast cancer, and its loss induces cell migration, invasiveness and tumor growth PMID: 21478912
  28. Data show that the AF6 RA1 domain efficiently mediated self-association, suggesting that constitutive MLL self-association is a more common pathogenic mechanism for MLL oncogenesis than indicated by previous studies of rare MLL fusion partners. PMID: 20395419
  29. Results demonstrate a novel molecular mechanism by which Rap1 and afadin regulate the VEGF- and S1P-induced angiogenesis. PMID: 20413783
  30. The results indicate that afadin has a novel role in PDGF-induced directional cell movement, presumably in cooperation with active Rap1 and SHP-2. PMID: 19887591
  31. Overexpressed nectin recruited N-cadherin DN to the nectin-based cell-cell adhesion sites in an afadin-dependent manner. PMID: 12686612
  32. nectins and afadin are involved in dynamic epithelial remodeling during mouse development PMID: 15108322
  33. afadin plays a critical role in the proper regulation of the PDGF-induced activation of PDGF receptor and signaling by Ras-ERK; this effect, which is mediated by SHP-2, impacts cellular morphology PMID: 17971444
  34. afadin organizes the formation of cell-cell junctions by regulating cell polarization in early embryonic development. PMID: 18173749
  35. These data indicate that the synaptic localization of nectin-1 and l-afadin are F-actin-dependent and that the shedding of nectin-1 is a mechanism contributing to synaptic plasticity. PMID: 18181141
  36. These results indicate that afadin is involved not only in the assembly of nectins and cadherins at synapses, but also in synaptic remodeling. PMID: 19481057
  37. afadin regulates the cyclical activation and inactivation of Rap1, Rac1, and RhoA through SPA-1 and ARAP1. PMID: 19589776

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Subcellular Location
Cell junction, adherens junction.
Tissue Specificity
Isoform 1 is expressed only in a restricted set of epithelial structures during early embryogenesis.
Database Links
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