Recombinant Mouse Agouti-signaling protein (Asip)

Code CSB-YP002212MO
MSDS
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Source Yeast
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Code CSB-EP002212MO
MSDS
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Source E.coli
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Code CSB-EP002212MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002212MO
MSDS
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Source Baculovirus
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Code CSB-MP002212MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Asip
Uniprot No.
Alternative Names
Asip; aAgouti-signaling protein; ASP; Agouti coat color protein; Agouti switch protein
Species
Mus musculus (Mouse)
Expression Region
23-131
Target Protein Sequence
HLALEETL GDDRSLRSNS SMNSLDFSSV SIVALNKKSK KISRKEAEKR KRSSKKKASM KKVARPPPPS PCVATRDSCK PPAPACCDPC ASCQCRFFGS ACTCRVLNPN C
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). Causes hair follicle melanocytes to synthesize phaeomelanin instead of black or brown pigment eumelanin and produces hairs with a subapical yellow band on an otherwise black or brown background when expressed during the mid-portion of hair growth.
Gene References into Functions
  1. The data confirm development of obesity and disturbance of lipid metabolism in Ay males. There were no changes in testicular function. PMID: 30188668
  2. Methylation analysis reveals that although most lines display normal methylation at IAP elements in general, the Avy IAP element is essentially unmethylated. PMID: 25191835
  3. observed variable histone patterns in the 5' long terminal repeat of the viable yellow agouti (A(vy)) metastable epiallele PMID: 20671424
  4. Data suggest that that the agouti/melanocortin signaling pathway regulates levels of peroxisome proliferator activated receptor gamma in adipocytes. PMID: 12851309
  5. AP overproduction increased the response of the pituitary adrenal system to short-restraint stress due to increased adrenal responsiveness to ACTH PMID: 15296483
  6. Local long-term mouse Agouti expression in the PVN, DMH, and LH differentially induced hyperphagia and accelerated body weight gain without apparent changes in central neuropeptide systems that are known to stimulate food intake in rats. PMID: 15537888
  7. mice with different agouti genotypes had identical basal corticosterone levels, though yellow mice, as compared with black mice, had increased corticosterone levels after restriction stress PMID: 15779332
  8. Corin plays a critical role in specifying coat color and acts downstream of agouti gene expression as a suppressor of the agouti pathway. PMID: 18057101
  9. The preimplantation embryo's growth environment can affect the postnatal expression of a defined epigenetically sensitive allele. PMID: 18562706
  10. pigment-related genes targeted by ASP and by alphaMSH PMID: 19174519
  11. study shows ASIP-MC1R signaling includes a cAMP-independent pathway through attractin and mahogunin, while the known cAMP-dependent component requires neither attractin nor mahogunin PMID: 19493315
  12. effects of the A(y) allele on mandible morphology on different strain backgrounds, DDD and B6 PMID: 19644225

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Subcellular Location
Secreted.
Tissue Specificity
Epithelial cells of the hair follicles and the epidermis.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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