Recombinant Mouse Alpha-defensin 1 (Defa1)

Code	CSB-YP006652MO
Abbreviation	Recombinant Mouse Defa1 protein
MSDS	MSDS Datasheet
Size	$368
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Defa1

Uniprot No.

P11477

Research Area

Immunology

Alternative Names

Defa1; Defcr; Defcr1Alpha-defensin 1; Cryptdin-1; Defensin-related cryptdin peptide

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

59-93aa

Target Protein Sequence

LRDLVCYCRSRGCKGRERMNGTCRKGHLLYTLCCR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

31.1kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The production of this Recombinant Mouse Defa1 protein required the insertion of a DNA fragment (Defa1, 59-93aa) into a plasmid vector and the transferral of this vector into Yeast cells (the step of transformation). The cells were then cultured and induced to express the Defa1 protein. This recombinant protein was fused with N-terminal GST tag. Its purity is 90%+ determined by SDS-PAGE.

Defa1 (also called Defcr or Defcr1) is a gene providing an instruction of making a protein named alpha-defensin 1 (Defa1) in mus musculus (mouse). Defa1 protein is also known as cryptdin-1 or defensin-related cryptdin peptide and belongs to alpha-defensin (α-defensin) family. Mammalian α-defensins are cationic antimicrobial peptides (AMPs) with microbicidal activities against many species of bacteria (such as gram-negative and gram-positive bacterium) and fungi, antiviral functions, and diverse immunomodulatory effects (such as innate immune response in mucosa).

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Target Background

Function

Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.

Gene References into Functions

Mouse adenovirus 2 infection was enhanced by enteric alpha-defensins. PMID: 28622386
Decreased alpha-defensin is associated with intestinal inflammation in a model of inflammatory bowel disease. PMID: 20222124
cryptdin-1 and cryptdin-4 exhibit circadian oscillation PMID: 16129697
the Arg5-Glu13 salt bridge in alpha-defensin is conserved for defensin in vivo stability PMID: 16246847
MMP-7 activates mouse paneth cell pro-alpha-defensins PMID: 16822871

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Subcellular Location

Secreted.

Protein Families

Alpha-defensin family

Tissue Specificity

Paneth cells of the small bowel.

Database Links

KEGG: mmu:13216

UniGene: Mm.425522

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