Recombinant Mouse Angiomotin(Amot) ,partial

Code CSB-YP001677MO
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Source Yeast
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Code CSB-EP001677MO
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Source E.coli
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Code CSB-EP001677MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001677MO
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Source Baculovirus
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Code CSB-MP001677MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Amot
Uniprot No. Q8VHG2
Alternative Names Amot; Kiaa1071Angiomotin
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions (By similarity).
Gene References into Functions
  1. Rho attenuates the interaction between Amot and Nf2 by binding to the coiled-coil domain of Amot. PMID: 28947533
  2. a new function of RNF146 and tankyrase in stabilizing the Crumbs complex through downregulation of AMOT proteins at the apical membrane, is reported. PMID: 27521426
  3. AMOT is a crucial suppressor of lung cancer metastasis and highlight its critical role as a tumor suppressor and its potential as a prognostic biomarker and therapeutic target for lung cancer. PMID: 25381822
  4. The loss of Angiomotin, together with Angiomotin-like 2, leads to differentiation of inner cell mass cells and compromised peri-implantation development. PMID: 23903990
  5. The phosphorylation of S176 in the N-terminal domain of Amot is a critical step for activation of the Hippo pathway in adherens junctions and cell polarity disconnects the Hippo pathway from cell-cell adhesion by sequestering Amot from AJs. PMID: 23791731
  6. Amot, Amotl1, and Amotl2 are differentially expressed in uterine cells during the peri-implantation period. PMID: 22813598
  7. A vaccine targeting angiomotin induces an antibody response which alters tumor vessel permeability and hampers the growth of established tumors. PMID: 22426512
  8. Depletion of Angiomotin in Nf2(-/-) Schwann cells attenuates the Ras-MAPK signaling pathway, impedes cellular proliferation in vitro and tumorigenesis in vivo PMID: 21481793
  9. Angiomotin may promote neoplastic angiogenesis by both stimulating invasion as well as stabilizing established tubes. PMID: 14730344
  10. angiomotin, in addition to controlling cell motility, may play a role in the assembly of endothelial cell-cell junctions PMID: 16043488
  11. p80- and p130-angiomotin play coordinating roles in vascular tube formation by affecting cell migration and cell shape, respectively. PMID: 16640563
  12. Data show that MUPP1 interacts with angiomotin (Amot), JEAP/Amot-like 1 and MASCOT/Amot-like 2, and that all the Amot/JEAP family proteins also interacted with Patj, a close relative of MUPP1. PMID: 17397395
  13. 75% of Amot knockout mice die between embryonic day 11 (E11) and E11.5 and exhibit severe vascular insufficiency in the intersomitic region as well as dilated vessels in the brain. PMID: 17699752
  14. Homo-oligomerization of p80-Amot and hetero-oligomerization of both isoforms are critical for a switch between a migratory and a non-migratory cell phenotype in endothelial cells. PMID: 18164266
  15. Amot and AmotL1 have similar effects on endothelial migration and tight junction formation in vitro. In vivo Amot appears to control the cell polarity and AmotL1 affects the stability of cell-cell junctions. PMID: 19590046

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Subcellular Location Cell junction, tight junction
Protein Families Angiomotin family
Tissue Specificity Expressed in brain, skeletal muscle and placenta.
Database Links

KEGG: mmu:27494

STRING: 10090.ENSMUSP00000108455

UniGene: Mm.100068

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