Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Target Names

Cspg4

Uniprot No.

Q8VHY0

Research Area

Cancer

Alternative Names

Chondroitin sulfate proteoglycan NG2;Proteoglycan AN2

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

1588-2229aa

Target Protein Sequence

SLEGTRKLTVCPESVQPLSSQSLSASSSTGADPRHLLYRVVRGPQLGRLLHAQQGSAEEVLVNFTQAEVNAGNILYEHEMSSEPFWEAHDTIGLLLSSPPARDLAATLAVMVSFDAACPQRPSRLWKNKGLWVPEGQRAKITVAALDAANLLASVPASQRSRHDVLFQVTQFPTRGQLLVSEEPLHARRPYFLQSELAAGQLVYAHGGGGTQQDGFRFRAHLQGPTGTSVAGPQTSEAFVITVRDVNERPPQPQASIPLRVTRGSRAPVSRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLAGDNTGPVTHFTQADVDAGRLAFVANGSSVAGVFQLSMSDGASPPIPMSLAVDVLPSTIEVQLRAPLEVPQALGRTSLSRQQLQVISDREEPDVAYRLTQGPLYGQLLVGGQPASAFSQLQVDQGDVVFVFTNFSSSQDHFKVVALARGVNASATVNVTVQALLHVWAGGPWPQGTTLRLDPTVLDASELANRTGSMPHFRLLAGPRYGRVVRVSQGRTESRSNQLVEHFTQRDLEEGQLGLEVGKPEGRSTGPAGDRLTLELWAKGVPPAVALLDFATEPYHAAKSYSVALLSVPEAVRTETEKPGRSVPTGQPGQAASSPVPTAAKGGFLGFLEANMFS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

70.1 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

Gene References into Functions

This study confirm the earlier report that EAE disease severity is ameliorated in the absence of NG2 proteoglycan, but do not give any indication of NG2 expression by immune cells in naive and immunized mice. PMID: 28567522
These findings suggest that NG2 expression mediates inflammatory reactions and neurodegeneration in microglial cells in response to central nervous system injury, potentially by regulating FAK phosphorylation. PMID: 27306838
NG2 may represent a promising target for the modulation of ICAM-1-mediated immune responses. PMID: 28964848
Results demonstrate that NG2 plays an important role in the induction and progression of experimental autoimmune encephalomyelitis. We further demonstrate that this role exerts itself at both the central nervous system/blood-brain barrier and immune system levels PMID: 27026411
Loss of chondroitin sulfate proteoglycan NG2 (NG2) does not affect oligodendroglial differentiation. PMID: 27755537
Study found significant differences in transcript and protein expression, electrophysiological properties and proliferative capacity of NG2 glia in the mouse forebrain, suggesting the co-existence of several subpopulations of NG2 glia. PMID: 27306788
chondroitin sulfate proteoglycan 4 (CSPG4) is a potential mesenchymal progenitor cells marker. PMID: 27582000
Results suggest that NG2 may counteract neurological deficits and adverse glial responses in traumatic brain injury PMID: 26638112
NG2 protein is not required for glutamatergic neuron-NG2 cell synaptic signaling PMID: 25100858
This study demonstrated that NG2 is an important factor in OPC-dependent regeneration of white matter following spinal cord demyelination. PMID: 26338007
NF-kappaB in NG2(+) cells promotes myoblast migration to the tips of myofibers through cell-cell contact through expression of ephrinA5 from NG2(+) cells PMID: 26777211
The NG2 proteoglycan protects oligodendrocyte precursor cells against oxidative stress via interaction with OMI/HtrA2. PMID: 26340347
NG2 ablation leads to reduced LDL sequestration in the aorta and reduced atherogenesis. PMID: 26543095
This study demonstrated that a pathway and role for NG2 glia in central nerve system homeostasis and mood disorders. PMID: 26606998
cleaved by ADAM10 and the gamma-secretase on oligodendrocyte precursor cells PMID: 25387269
The results of this study indicated that endogenous netrin-1 plays a role in NG2(+) glial cell homeostasis that is distinct from its role in myelination. PMID: 25926469
The study demonstrates changes in pericyte expression of NG2 and PDGFRB and vascular permeability in the sensory circumventricular organs by chronic osmotic stimulation. PMID: 23629811
NG2 knockdown results in loss of beta1 integrin activation in endothelial cells, revealing a mechanism for NG2-dependent cross talk between pericytes and endothelial cells. PMID: 23925489
Data show that neurofibromatosis type 1 (NF1)-inactivation results in a cell-autonomous increase in glial fibrillary acidic protein+ (GFAP+), but not in NG2 proteoglycan NG2+, cell proliferation in vitro. PMID: 23318450
As soon as cells exit mitosis, a fraction of NG2-expressing cells displays accumulation of G protein-coupled receptor (GPR)17 protein in the Golgi apparatus. PMID: 21956849
Traumatic brain injury results in an increase in the chondroitin sulfate proteoglycans (CSPGs) neurocan and NG2 expression in a tight band surrounding the injury core. PMID: 22628090
the importance of NG2 in mediating pericyte/endothelial cell communication that is required for proper vessel maturation and function PMID: 22531600
brown fat dysfunction in NG2 null mice results from deficits in the recruitment and/or development of brown pre-adipocytes PMID: 22295099
These studies identify NG2 as an important factor in regulating myelin processing. PMID: 22078261
Overexpression of CSPG4 is associated with tumour growth and angiogenesis in glioblastoma multiforme and melanoma PMID: 21829586
Results suggest that NG2 cells are unlikely to play an inhibitory boundary role on TCA growth and that NG2 expression is not necessary for normal barrel formation. PMID: 21292011
PDGF-B-driven gliomagenesis can occur in the absence of the proteoglycan NG2 PMID: 20939912
live cell imaging of single NG2 cells from early postnatal brain slices revealed that NG2 cells initially divide symmetrically to produce two daughter NG2 cells and that differentiation into oligodendrocytes occurred after 2-3 days PMID: 21266410
NG2 expressing cells constitute the major immature population of the mouse cortex, since not only oligodendrocyte precursor cells and pre-oligodendrocytes but also a part of the glial-restricted progenitors express the NG2 proteoglycan. PMID: 21072997
a stable population of quiescent NG2 cells appears before the end of the first postnatal week and persists throughout life PMID: 20346197
NG2 may promote OPC proliferation via enhancement of growth factor signaling or mediation of OPC interaction with unmyelinated axons. PMID: 20006679
a population of cortical NG2(+) cells display physiological properties that differ from their white matter counterparts. PMID: 15358811
dendritic cell-based immunization represents an effective strategy to implement T cell-based immunotherapy targeting high molecular weight-melanoma-associated antigen (HMW-MAA) in patients with HMW-MAA-bearing tumors [HMW-MAA] PMID: 16455987
The NG2 null epidermis does not achieve its full thickness due to reduced proliferation of basal keratinocytes that serve as the stem cell population in this layer. Thickening of the subcutis is delayed due to deficiencies in the adipocyte population. PMID: 18040080
syntenin plays a role in the migration of oligodendroglial precursors, and we suggest that NG2-syntenin-1 interactions contribute to this PMID: 18218632
mice expressed the NG2 protein in all regions and at all ages studied PMID: 18924152
These observations indicate that NG2 cells serve as precursor cells for oligodendrocytes and a subpopulation of protoplasmic astrocytes throughout the rostrocaudal axis of the CNS. PMID: 19006598
Results indicate that NG2 cells do not represent neuronal progenitor cells in the postnatal SVZ but are likely to be oligodendrocyte precursor cells. PMID: 19058188
murine GL261 cerebral gliomas express AN2 and HMP-specific mAb VT68.2 accumulates selectively and specifically at high concentration and is retained within murine cerebral gliomas PMID: 19152070

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Subcellular Location

Cell membrane; Single-pass type I membrane protein; Extracellular side. Apical cell membrane; Single-pass type I membrane protein; Extracellular side. Cell projection, lamellipodium membrane; Single-pass type I membrane protein; Extracellular side. Cell surface.

Tissue Specificity

Expressed in microcascular pericytes and not endothelial cells.

Database Links

KEGG: mmu:121021

STRING: 10090.ENSMUSP00000038909

UniGene: Mm.41329

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Code	CSB-YP006076MO
MSDS	MSDS
Size	$368
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Chondroitin sulfate proteoglycan 4 (Cspg4), partial

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