Recombinant Mouse Collagen alpha-1 (VII) chain (Col7a1), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Col7a1

Uniprot No.

Q63870

Alternative Names

Col7a1Collagen alpha-1(VII) chain; Long-chain collagen; LC collagen

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Gene References into Functions

Biophysical characterization of a pathogenic type VII collagen mutant is presented. The pathogenic mutant lowers thermal stability of the type VII collagen domain. Due to unfolding, protease cleavage sites in the domain become accessible. PMID: 27989960
specifically binds and sequesters the innate immune activator cochlin in the lumen of lymphoid conduits PMID: 29305555
miR-29 Regulates COL7A1 in Recessive Dystrophic Epidermolysis Bullosa, directly through targeting its 3' untranslated region at two distinct seed regions and indirectly through targeting an essential transcription factor required for basal COL7A1 expression, SP1 PMID: 27328306
autoantibodies to COL7, independent of the targeted epitopes, induce blisters both ex vivo and in vivo PMID: 25689103
The cystine knot is N-terminal to the collagen triple helix in in collagen type VII and IX and therefore probably impedes unfolding of the collagen triple helix from the N terminus. PMID: 24385431
COL7A1 is required for re-epithelialization through organization of laminin-332 at the dermal-epidermal junction during wound healing. PMID: 23867500
COL7 regulates ameloblast differentiation and is essential for the formation of Tomes' processes. PMID: 22940071
Tissue-bound, but not circulating, complement-fixing COL7-specific autoantibodies are associated with T helper type (Th)1 cell type cytokine expression and response in mice seusceptible to epidermolysis bullosa acquisita. PMID: 21967893
An interaction of mvWFA2 with its neighboring domain mFNIII-9 was characterized with NMR spectroscopy and SPR PMID: 21570975
transforming growth factor beta1 stimulation of Col7a1 transcription is dependent on a putative interaction between Smads and AP1. PMID: 14675198
single amino acid substitutions in procollagen VII alter its self-assembly PMID: 15509587
All mice developed circulating IgG autoantibodies that recognized type VII collagen and bound to the lamina densa of the dermal-epidermal junction[type VII collagen] PMID: 16920988
Results describe the generation of a collagen VII alpha 1 hypomorphic mouse that serves as an immunocompetent animal model for dystrophic epidermolysis bullosa. PMID: 18382769

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Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Database Links

KEGG: mmu:12836

STRING: 10090.ENSMUSP00000026740

UniGene: Mm.6200

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Recombinant Mouse Collagen alpha-1 (VII) chain (Col7a1), partial

Product Details

Customer Reviews and Q&A

Target Background

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