Recombinant Mouse Collagen triple helix repeat-containing protein 1 (Cthrc1)

Code CSB-BP875181MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-EP875181MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cthrc1
Uniprot No.
Alternative Names
Cthrc1; Collagen triple helix repeat-containing protein 1
Species
Mus musculus (Mouse)
Expression Region
33-245
Target Protein Sequence
SENPKVKQ KALIRQREVV DLYNGMCLQG PAGVPGRDGS PGANGIPGTP GIPGRDGFKG EKGECLRESF EESWTPNYKQ CSWSSLNYGI DLGKIAECTF TKMRSNSALR VLFSGSLRLK CRNACCQRWY FTFNGAECSG PLPIEAIIYL DQGSPELNST INIHRTSSVE GLCEGIGAGL VDVAIWVGTC SDYPKGDAST GWNSVSRIII EELPK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May act as a negative regulator of collagen matrix deposition.
Gene References into Functions
  1. CTHRC1 secreted from osteocytes and osteoblasts functions as an inhibitor of osteoclast differentiation via inhibition of NFkappaB-dependent signaling. PMID: 28115279
  2. These data suggest Cthrc1 reduces fibrotic tissue formation in bleomycin-induced lung fibrosis and the effect is potent enough to limit the decline in lung function. PMID: 28292882
  3. CTHRC1 was established as a novel marker of activated synoviocytes in murine experimental arthritis and rheumatoid arthritis PMID: 27430622
  4. CTHRC1 is an osteoclast-secreted coupling factor that regulates bone remodeling. PMID: 23908115
  5. This study demonstrated that Cthrc1 plays a negative regulatory role, fine-tuning the onset of peripheral myelination. PMID: 22379615
  6. this is the first demonstration of Cthrc1 as a marker of the severity of the disease progression in the dystrophic muscles. PMID: 23274062
  7. Hormonal functions of Cthrc1 include regulation of lipid storage and cellular glycogen levels with potentially broad implications for cell metabolism and physiology. PMID: 23056600
  8. Data show a dramatic discrepancy between ROSA26 reporter activity and Pdgfrb promoter driven Cre dependent myc-tagged Cthrc1 transgene expression. PMID: 21557454
  9. Downregulated by dietary beta-carotene in lungs of knockout Bcmo1-deficient mice. PMID: 20472610
  10. Cthrc1 expression overlaps considerably with those reported for TGF-beta family members and interstitial collagens. PMID: 16678498
  11. Cthrc1 selectively activates the planar cell polarity pathway of Wnt signaling by stabilizing the Wnt-receptor complex. PMID: 18606138
  12. Cthrc1 increases bone mass as a positive regulator of osteoblastic bone formation PMID: 18779865

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Database Links
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