Product Details

Purity

>85% (SDS-PAGE)

Target Names

Mlh1

Uniprot No.

Q9JK91

Alternative Names

Mlh1; DNA mismatch repair protein Mlh1; MutL protein homolog 1

Species

Mus musculus (Mouse)

Expression Region

1-760

Target Protein Sequence

MAFVAGVIRR LDETVVNRIA AGEVIQRPAN AIKEMIENCL DAKSTNIQVV VKEGGLKLIQ IQDNGTGIRK EDLDIVCERF TTSKLQTFED LASISTYGFR GEALASISHV AHVTITTKTA DGKCAYRASY SDGKLQAPPK PCAGNQGTLI TVEDLFYNII TRRKALKNPS EEYGKILEVV GRYSIHNSGI SFSVKKQGET VSDVRTLPNA TTVDNIRSIF GNAVSRELIE VGCEDKTLAF KMNGYISNAN YSVKKCIFLL FINHRLVESA ALRKAIETVY AAYLPKNTHP FLYLSLEISP QNVDVNVHPT KHEVHFLHEE SILQRVQQHI ESKLLGSNSS RMYFTQTLLP GLAGPSGEAA RPTTGVASSS TSGSGDKVYA YQMVRTDSRE QKLDAFLQPV SSLGPSQPQD PAPVRGARTE GSPERATRED EEMLALPAPA EAAAESENLE RESLMETSDA AQKAAPTSSP GSSRKRHRED SDVEMVENAS GKEMTAACYP RRRIINLTSV LSLQEEISER CHETLREMLR NHSFVGCVNP QWALAQHQTK LYLLNTTKLS EELFYQILIY DFANFGVLRL SEPAPLFDLA MLALDSPESG WTEDDGPKEG LAEYIVEFLK KKAEMLADYF SVEIDEEGNL IGLPLLIDSY VPPLEGLPIF ILRLATEVNW DEEKECFESL SKECAMFYSI RKQYILEEST LSGQQSDMPG STSKPWKWTV EHIIYKAFRS HLLPPKHFTE DGNVLQLANL PDLYKVFERC

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Heterodimerizes with Pms2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (Msh2-Msh6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of Pms2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (Mlh1-Pms2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with Mlh3 to form MutL gamma which plays a role in meiosis.

Gene References into Functions

high mutation ofMlh1(-/-)-deficient fetuses has little effect on the fetuses during their early developmental stages, whereas Mlh1(-/-)-deficient fetuses from X-ray irradiated mothers are clearly effected PMID: 27643877
radiation exposure could further increase the risk of colorectal carcinogenesis induced by inflammation under the conditions of Mlh1 deficiency. PMID: 25529563
these data identify Mlh1 and Mlh3 as novel critical genetic modifiers of HTT CAG instability, point to Mlh1 genetic variation as the likely source of the instability difference in B6 and 129 strains PMID: 24204323
Data indicate that Mlh1 showed only modest methylation was still expressed in both Mlh1(+/-) and Mlh1(+/+) mice. PMID: 24204690
nickel-smelting fumes upregulated the expression of Mlh1 protein, mouse . This suggest that nickel-smelting fumes could be toxic to cells, inducing cell apoptosis and necrosis. PMID: 24579805
suggesting a role for the ATPase activity of MLH1 beyond the activation of the endonuclease functions of its MMR partner PMS2 PMID: 22451719
Down-regulation of MLH1 is associated with initiation and growth of neuroblastoma and brain tumour multicellular spheroids. PMID: 22145025
MLH1 can convert DNA nicks and point mutations into double-stranded DNA breaks for both core nonhomologous end-joining factors and alternative end-joining pathways of class-switch recombination. PMID: 21242524
Data show that the constitutive inactivation of MLH1, resulting Mlh1(Deltaex4/Deltaex4) mouse line, displays complete MMR deficiency and a cancer predisposition phenotype similar to Mlh1-/- mice. PMID: 20858091
Ablation of Mlh1 did not appreciably influence the developmental phenotypes conferred by the absence of Rad51d. PMID: 20133210
Role for mismatch repair proteins Msh2, Mlh1, and Pms2 in immunoglobulin class switching shown by sequence analysis of recombination junctions. PMID: 11828012
In this study, we investigated whether failure to develop foci of MLH1 protein on chromosomes in prophase would lead to elimination of prophase spermatocytes.Thus, spermatocytes are not eliminated in direct response to the initial meiotic defect PMID: 12217320
We found that MLH1 and PMS2 have functional nuclear localization signals (NLS) and nuclear export sequences, yet nuclear import depended on their C-terminal dimerization to form MutLalpha PMID: 12697830
Mlh1 can function in antibody class switch recombination independently of Msh2. PMID: 12743174
Mlh1 deficiency accelerates myeloid leukemogenesis in neurofibromatosis 1 (Nf1) heterozygous mice.These results suggest that MMR deficiency can accelerate myeloid leukemogenesis in Nf1+/- mice, presumably by inactivating Nf1 gene expression. PMID: 12881715
The cellular content of MLH1 protein seems to be critical for determining if damaged cells enter into either a death or mutation-inducing pathway. PMID: 13679151
Mlh1 modulates error-prone nonhomologous end-joining by inhibiting the annealing of DNA ends containing noncomplementary base pairs or by promoting the annealing of microhomologies. PMID: 15084308
Repression of MLH1 is associated with precancerous conditions in hyperplastic mucosa adjacent to colon cancer PMID: 15354418
role in regulating mitotic recombination PMID: 15480418
Comparison of DES-treated and untreated Mlh1-/- animals suggests the combination of Mlh1 deficiency and diethylstilbestrsol exposure accelerates lymphomagenesis. PMID: 15700306
Mlh1-deficient mice and Msh2-deficient mice had the highest mutation frequencies in a comparison of the single nullizygous mice. PMID: 16728433
Ikaros mutations coupled with Mlh1 deficiency in lymphomagenesis. PMID: 17086208
One or two TNs of the 10-15 TNs per SC acquire MLH1 protein, the hallmark of reciprocal recombination, whereas the TNs that do not acquire MLH1 protein relocate from their positions along the midline of the SCs to the periphery of the SCs. PMID: 17344431
Mice with prostate-specific MLH1 deletion demonstrated more rapid carcinoma transformation of the prostate. PMID: 17619846
Meiotic progression in female mice bearing mutations in genes of the DNA mismatch repair pathway. PMID: 18057311
ATPase activity of Mlh1 is essential for fertility in mammals PMID: 18337503
MLH1-independent crossovers in mammalian meiosis,in Mus81-nullizygous mice. PMID: 18787696
Mlh1 or Exo1 deficiencies also eliminate class switch recombination in the absence of the SmuTR PMID: 19553545
Treating Gialpha2-/- mice with the histone deacetylase inhibitor suberoylanilide hydroxamic acid significantly decreased colitis activity and rescued MLH1 expression in crypt epithelial cells. PMID: 19638594
Mice with deficiencies in both Chfr and Mlh1 (the gene that encodes the DNA mismatch-repair protein Mlh1) displayed dramatically higher incidence of spontaneous tumors relative to mice deficient for only one of these genes. PMID: 19690386
sulindac increased tumors in the small intestine of Mlh1 mutant mice PMID: 19755659

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Subcellular Location

Nucleus. Chromosome.

Protein Families

DNA mismatch repair MutL/HexB family

Database Links

KEGG: mmu:17350

STRING: 10090.ENSMUSP00000035079

UniGene: Mm.486383

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Recombinant Mouse DNA mismatch repair protein Mlh1 (Mlh1)

Product Details

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Target Background

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