Recombinant Mouse DNA polymerase kappa (Polk), partial

Code CSB-YP889539MO
MSDS
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Source Yeast
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Code CSB-EP889539MO
MSDS
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Source E.coli
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Code CSB-EP889539MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP889539MO
MSDS
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Source Baculovirus
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Code CSB-MP889539MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Polk
Uniprot No.
Alternative Names
Polk; Dinb1; DNA polymerase kappa; EC 2.7.7.7; DINB protein; DINP
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Gene References into Functions
  1. The results suggest that Polk has a limited ability to suppress BP-induced genotoxicity in the colon and bone marrow and also that the roles of specialized DNA polymerases in mutagenesis and carcinogenesis should be examined not only by in vitro assays but also by in vivo mouse studies. We also report the spontaneous mutagenesis in inactivated Polk KI mice at young and old ages. PMID: 29076178
  2. the extreme N-terminal part of Polkappa is required for the processivity and fidelity of Polkappa during translesion synthesis of 10S(+)-trans-anti-benzo[a]pyrene diol epoxide-N(2)-deoxyguanine adducts lesions. PMID: 26634445
  3. Polk plays a predominant role in suppressing point mutations by carrying out error-free translesion DNA synthesis and contributes to the prevention of DNA strand breaks. PMID: 25303778
  4. The structural gap physically accommodates the bulky aromatic adduct and the N-clasp is essential for the structural integrity and flexibility of Polkappa during translesion synthesis. PMID: 24449898
  5. Polkappa accumulates at laser-induced sites of DNA damage. PMID: 23522793
  6. Structural basis of Rev1-mediated assembly of a quaternary vertebrate translesion polymerase complex consisting of Rev1, heterodimeric polymerase (Pol) zeta, and Pol kappa PMID: 22859295
  7. Data suggest that DNA polymerase kappa Polkappa functions in DNA interstrand crosslinks (ICLs) repair in embryonic fibroblast cells (MEF), especially during the G0/G1 phases. PMID: 22658724
  8. solution structure of the polymerase kappa-Rev1 complex PMID: 22700975
  9. results are consistent with the notion that Pol kappa is required for accurate translesion DNA synthesis past naturally occurring polycyclic guanine adducts, possibly generated by cholesterol and/or its metabolites. PMID: 19783230
  10. Polkappa plays an important role in suppressing mutations at DNA lesions generated by benzo[a]pyrene, but not UV or x-ray irradiation. PMID: 12432099
  11. Rev7 competes directly with Pol kappa for binding to the Rev1 C-terminus. PMID: 14657033
  12. The multiple mouse/human (PolK/POLK) transcripts may encode multiple Polkappa isoforms in testis PMID: 15661663
  13. the trans-lesion synthesis enzyme polkappa is specifically required for normal recovery from the BPDE-induced S-phase checkpoint PMID: 15817457
  14. Polk causes error-prone and inefficient replication across 8-oxoguanine in ras genes. PMID: 15938713
  15. Polkappa has a function in translesion DNA synthesis past alkylated base adducts as well as UV radiation DNA damage in vertebrates PMID: 16308320
  16. compromised translesion synthesis in Polkappa(-/-) mice may result in replication fork pausing which, in turn, may affect expanded simple tandem repeat mutation rate PMID: 16731053
  17. pol kappa-deficient mouse cells have substantially reduced (but not absent) levels of nucleotide excision repair PMID: 16738703
  18. Polkappa binds with monoubiquitinated proliferating cell nuclear antigen (PCNA) more robustly than with non-ubiquitinated PCNA. PMID: 18162470
  19. The REV1-interaction is essential for Polkappa function in vivo. PMID: 19170759

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Subcellular Location
Nucleus.
Protein Families
DNA polymerase type-Y family
Tissue Specificity
Detected at low levels in heart, brain, lung, liver, kidney and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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