Recombinant Mouse DNA polymerase lambda (Poll)

Code CSB-YP874105MO
MSDS
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Source Yeast
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Code CSB-EP874105MO
MSDS
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Source E.coli
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Code CSB-EP874105MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874105MO
MSDS
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Source Baculovirus
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Code CSB-MP874105MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Poll
Uniprot No.
Alternative Names
Poll; PolkDNA polymerase lambda; Pol Lambda; EC 2.7.7.7; EC 4.2.99.-; DNA polymerase kappa
Species
Mus musculus (Mouse)
Expression Region
1-573
Target Protein Sequence
MDPQGIVKAF PKRKKSHADL SSKALAKIPK REVGEARGWL SSLRAHIMPA GIGRARAELF EKQIIHHGGQ VCSAQAPGVT HIVVDEDMDY ERALRLLRLP QLPPGAQLVK STWLSLCLQE GRLTDTEGFS LPMPKRSLDE PQPSKSGQDA SAPGTQRDLP RTTLSLSPPH TRAVSPPPTA EKPSRTQAQL SSEDETSDGE GPQVSSADLQ ALITGHYPTP PEEDGGPDPA PEALDKWVCA QPSSQKATNY NLHITEKLEV LAKAYSVQGD KWRALGYAKA INALKSFHKP VSSYQEACSI PGIGKRMAEK VMEILESGHL RKLDHISDSV PVLELFSNIW GAGTKTAQMW YHQGFRNLED LQSLGSLTAQ QAIGLKHYDD FLDRMPREEA AEIEQTVRIS AQAFNPGLLC VACGSYRRGK MTCGDVDVLI THPDGRSHRG IFSCLLDSLR QQGFLTDDLV SQEENGQQQK YLGVCRLPGP GKRHRRLDII VVPYCEFACA LLYFTGSAHF NRSMRALAKT KGMSLSEHAL SAAVVRNSQG VKVGPGQVLP TPTEKDVFKH LGLPYREPAE RDW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
Gene References into Functions
  1. Results show that deficiency of either DNA polymerases beta or lambda or both results in a modest but significant decrease in V region somatic hypermutation (SHM) with no effect on mutation specificity, suggesting no direct role in SHM. PMID: 24084171
  2. both pol lambda and pol beta interact with the upstream DNA glycosylases for repair of alkylated and oxidized DNA bases PMID: 20805875
  3. analysis of the interaction between DNA Polymerase lambda and anticancer nucleoside analogs PMID: 20348107
  4. Hydrocephalus, situs inversus, chronic sinusitis, and male infertility in DNA polymerase lambda-deficient mice: possible implication for the pathogenesis of immotile cilia syndrome. PMID: 11909969
  5. Pol lambda is not required for normal Ig gene hypermutation. PMID: 11937519
  6. Pol lambda contributes to base excision repair in mouse fibroblast cell extract. PMID: 15749700
  7. Pol lambda protects cells against oxidative stress, and participates in oxidative DNA damage base excision repair. PMID: 16002405

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Subcellular Location
Nucleus.
Protein Families
DNA polymerase type-X family
Database Links
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