Recombinant Mouse Diablo homolog, mitochondrial (Diablo)

Code CSB-YP878067MO
MSDS
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Source Yeast
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Code CSB-EP878067MO
MSDS
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Source E.coli
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Code CSB-EP878067MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878067MO
MSDS
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Source Baculovirus
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Code CSB-MP878067MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Diablo
Uniprot No.
Alternative Names
Diablo; SmacDiablo homolog; mitochondrial; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; Smac
Species
Mus musculus (Mouse)
Expression Region
54-237
Target Protein Sequence
AVPIAQK SEPQSLSNEA LMRRAVSLVT DSTSTFLSQT TYALIEAITE YTKAVYTLVS LYRQYTSLLG KMNSQEEDEV WQVIIGARVE MTSKQQEYLK LETTWMTAVG LSEMAAEAAY QTGADQASIT ARNHIQLVKS QVQEVRQLSQ KAETKLAEAQ TKELHQKAQE VSDEGADQEE EAYLRED
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases.
Gene References into Functions
  1. We report that an Smac-mimetic selectively induces TNF-alpha-dependent cystic renal epithelial cell death, leading to the removal of cystic epithelial cells from renal tissues and delaying cyst formation. PMID: 23990677
  2. Results demonstrate an essential and apoptosis-independent function of SMAC in tumor suppression and provide new insights into the biology and targeting of colon cancer. PMID: 22751125
  3. Loss of Smac/DIABLO alters both caspase-dependent and caspase-independent intrinsic programmed cell death. PMID: 21597464
  4. Membrane-associated XIAP induces mitochondrial outer membrane permeabilization leading to cytochrome c and Smac release, which is dependent on Bax and Bak. PMID: 19875445
  5. data suggest the existence of a redundant molecule or molecules capable of compensating for a loss of Smac/DIABLO function. PMID: 11971981
  6. mitochondrial activation by Bid is required for reversing the inhibitor-of-apoptosis proteins inhibition through Smac release PMID: 12011074
  7. Diablo was present within the mitochondria of healthy cells, but released into the cytosol following the induction of apoptosis by UV. PMID: 12058276
  8. Transient focal ischemia was produced for 1 hour in mice. Mitochondrial levels of DIABLO increased after 2-11 h reperfusion. DIABLO increased in the cytosol after 5 h reperfusion, implying the translocation of DIABLO into the cytosol. PMID: 12395105
  9. Bax and Bak differentially regulate the release of cytochrome c and Smac/DIABLO from mitochondria, and Smac/DIABLO can be used to sensitize cells that are deficient in Bax and Bak genes, or resistant to TRAIL. PMID: 12670926
  10. Regulation of Smac/Diablo mRNA and protein expression is a mechanism by which lethal stimuli amplify their lethal potential in renal cells. PMID: 12969128
  11. These results suggest that interaction among XIAP, Smac/DIABLO, and caspase-9 plays an important role in the regulation of apoptotic neuronal cell death after transient focal cerebral ischemia. PMID: 12973017
  12. Smac/DIABLO is involved in the fragmentation and apoptosis of preimplantation embryos. PMID: 15709158
  13. release of Smac/DIABLO from mitochondria to cytoplasm and the subsequent activation of caspase-9 and caspase-3 play important roles in H(2)O(2)-induced apoptosis in C2C12 myogenic cells PMID: 16085184
  14. Smac/DIABLO and Hsp-70 proteins were upregulated 2-fold and 1.5-3-fold, respectively, after birth. PMID: 17914183

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Subcellular Location
Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis.
Tissue Specificity
Highest expression found in heart, liver, kidney and testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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