Product Details

Purity

≥90% as determined by SDS-PAGE.

Activity

Not Test

Target Names

Dusp4

Uniprot No.

Q8BFV3

Research Area

Signal Transduction

Alternative Names

Dusp4

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

2-398aa

Target Protein Sequence

VTMEELREMDCSVLKRLMNRDENGGGGSAGGNGSGSHGALGLLSGGKCLLLDCRPFLAHSAGYIRGSVNVRCNTIVRRRAKGSVSLEQILPAEEEVRARLRSGLYSAVIVYDERSPRAESLREDSTVSLVVQALRRNAERTDICLLKGGYERFSSEYPEFCSKTKALAAIPPPVPPSTNESLDLGCSSCGTPLHDQGGPVEILPFLYLGSAYHAARRDMLDALGITALLNVSSDCPNHFEGHYQYKCIPVEDNHKADISSWFMEAIEYIDAVKDCRGRVLVHCQAGISRSATICLAYLMMKKRVRLEEAFEFVKQRRSIISPNFSFMGQLLQFESQVLTTSCAAEAASPSGPLRERGKATPTPTSQFVFSFPVSVGVHAAPSNLPYLHSPITTSPSC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

50.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Regulates mitogenic signal transduction by dephosphorylating both Thr and Tyr residues on MAP kinases ERK1 and ERK2.

Gene References into Functions

Data provide evidence that repression of the ERK inhibitor DUSP4 by BMI1 is dependent on a more accessible chromatin configuration in G4 MB cells with low CHD7 expression. PMID: 29212025
study suggests that MKP-2 is essential to the pathogenic response of EAE, and it acts mainly via regulating the important antigen presenting DC function and T cell activation. PMID: 27958388
domain-mapping results showed that both the substrate-interacting and the phosphatase domains of DUSP4 were required for its optimal interaction with STAT5, while the coiled-coil domain of STAT5 appeared to hinder this interaction PMID: 26710253
MKP-2 knock-out mice show deficits in working memory and spatial reference. PMID: 26911683
DUSP4 is crucial for neuronal differentiation and functions in the neurogenesis of embryonic stem cells. PMID: 25397900
MKP-1 and MKP-2 stability is regulated by ERK-mediated phosphorylation through a degradation pathway independent of polyubiquitination PMID: 25204653
Loss of MKP-2 expression is associated with enhanced susceptibility to parasite infection. PMID: 23966857
LH/hCG tightly regulates MKP-2 expression, which modulates the induction of CYP11A1 by 8Br-cAMP. PMID: 23471219
Dusp1 and Dusp4 are cardioprotective genes that play a critical role in the heart by dampening p38 MAPK signaling that would otherwise reduce contractility and induce cardiomyopathy. PMID: 22993413
Loss of MKP-2 regulates early inflammation in acute lung injury. PMID: 22683570
Increased DUSP4 expression in activated T cells in the elderly in part accounts for defective adaptive immune responses. PMID: 22434910
protein deficiency enhances CD25 expression and CD4+ T-cell proliferation without impeding T-cell development PMID: 22101742
a critical non-redundant role for MKP-2 in regulating cell cycle progression and apoptosis. PMID: 21317287
These data support a mechanism by which MKP-2 targets ERK deactivation, thereby decreasing MKP-1 and thus removing the negative inhibition of MKP-1 on cytokine production. PMID: 20351138
E2F-1 is a transcriptional activator of MKP-2 and MKP-2 is an essential cell death mediator in the E2F-1 pathway PMID: 17452331
MKP-2 may play a crucial role in oncogenic signaling downstream of mutant RET, leading to deregulation of cell cycle. PMID: 18542059
Mediates transforming growth factor beta-dependent apoptosis by linking SMAD3 to the modulation of extracellular signal-regulated mitogen-activated protein kinase activity and pro-apoptotic events. PMID: 18704116

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Subcellular Location

Nucleus.

Protein Families

Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily

Database Links

KEGG: mmu:319520

STRING: 10090.ENSMUSP00000033930

UniGene: Mm.170276

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Code	CSB-EP807324MO(A4)
Abbreviation	Recombinant Mouse Dusp4 protein
MSDS	MSDS Datasheet
Size	$256
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Dual specificity protein phosphatase 4 (Dusp4)

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