Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
Fpr2; Fpr-rs2; Formyl peptide receptor 2; Formylpeptide receptor-related sequence 2; Lipoxin A4 receptor-like protein; N-formylpeptide receptor-like 2
Species
Mus musculus (Mouse)
Target Protein Sequence
MESNYSIHLNGSEVVVYDSTISRVLWILS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-GST-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression vector recombined with the recombinant DNA was transformed into the E.coli for expression. The recombinant DNA resulted from the fusion of the gene coding for the 1-29aa of the mouse Fpr2 protein and the N-terminal 10xHis-GST tag and C-terminal Myc tag gene. The product was purified and isolated to get the recombinant mouse Fpr2 protein with N-terminal 10xHis-GST tag and C-terminal Myc tag. This recombinant Fpr2 protein's purity reaches up to 85%. Under SDS-PAGE condition, this recombinant Fpr2 protein showed a band with a molecular weight of about 30-40 kDa on the gel.
Fpr2 is a gene encoding an enzyme named formyl peptide receptor 2(abbreviated Fpr2) and belongs to G-protein coupled receptor 1 family located on the surface of many cell types of various animal species. The formyl peptide receptors (FPRs) play an important functional role in host defense and inflammation by regulating the activation of phagocytes. Fpr2 is primarily expressed in neutrophils and not detected in vomeronasal neurons. An increasing body of evidence suggests that FPR2 plays a key role in immune resolution. It has been reported that FPR2 is activated by various ligands, such as pro-resolving eicosanoids (lipoxins and resolvins), and a synthetic hexapeptide, Trp-Lys-Tyr-Met-Val-D-Met-NH2 (WKYMVm).