Recombinant Mouse GPI-linked NAD (P) (+)--arginine ADP-ribosyltransferase 1 (Art1)

Code CSB-YP733724MO
MSDS
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Source Yeast
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Code CSB-EP733724MO
MSDS
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Source E.coli
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Code CSB-EP733724MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP733724MO
MSDS
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Source Baculovirus
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Code CSB-MP733724MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Art1
Uniprot No.
Alternative Names
Art1; Art2; GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1; EC 2.4.2.31; ADP-ribosyltransferase C2 and C3 toxin-like 1; ARTC1; Mono(ADP-ribosyl)transferase 1; YAC-1; CD antigen CD296
Species
Mus musculus (Mouse)
Expression Region
23-290
Target Protein Sequence
QSYSISQL DIFSQETPLD MAPASFDDQY AGCLADMTAA LPDLNHSEFQ ANKVYADGWA QANNQWQERR AWGSVWGSLP PSPPGFRDEH GVALLAYTAN SPLHKEFNAA VREAGRSRAH YLHHFSFKTL HFLLTEALQL LRSHRSRGCQ QVYRGVHGLR FRPAGPGATV RLGGFASASL KNVAAQQFGE DTFFGIWTCL GAPIRGYSFF PEEEEVLIPP FETFQVINTS RPTQGPARIY LRALGKRSTY NCEYIKEKKC RSGPCWLGSS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has ADP-ribosyltransferase activity toward GLP1R.
Gene References into Functions
  1. The results demonstrated that ART1 gene silencing inhibited the growth of the spleen transplanted tumor and its ability to spread to the liver via metastasis. PMID: 28138708
  2. data suggest that ART1 promoted the expression of HIF-1alpha via the Akt pathway in tumor cells. PMID: 26847718
  3. the apoptosis rate in the ART1-cDNA CT26 cells treated with PARP-1 inhibitor 5-aminoisoquinoline (5-AIQ) and cisplatin increased, when compared with the ART1-cDNA CT26 cells treated with cisplatin only or the untreated ART1-cDNA CT26 cells. PMID: 24676941
  4. ART1 knockdown reduced survival rate correlated with reduced levels of phos-Akt(Thr308) and phos-IkappaBalpha and reduced NF-kappaB p65 nuclear translocation. PMID: 24335275
  5. Successive amino- or carboxyl-terminal truncations of ART1, an arginine-specific transferase, identified regions that regulated transferase and NAD glycohydrolase activities. PMID: 12721285

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Subcellular Location
Sarcoplasmic reticulum membrane; Lipid-anchor, GPI-anchor.
Protein Families
Arg-specific ADP-ribosyltransferase family
Tissue Specificity
Abundantly expressed in cardiac and skeletal muscle. Low levels also found in lung.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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