Recombinant Mouse Gamma-aminobutyric acid receptor-associated protein(Gabarap)

Code CSB-YP872057MO
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Source Yeast
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Code CSB-EP872057MO
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Source E.coli
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Code CSB-EP872057MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872057MO
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Source Baculovirus
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Code CSB-MP872057MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Gabarap
Uniprot No. Q9DCD6
Alternative Names Gabarap; Gamma-aminobutyric acid receptor-associated protein; GABA(A) receptor-associated protein
Species Mus musculus (Mouse)
Expression Region 1-116
Target Protein Sequence MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYG
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Also required for the local activition of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction. Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation. Involved in apoptosis.
Gene References into Functions
  1. GABAergic synapse is proposed as a focus of aging. (Review) PMID: 28497576
  2. Cells that lacked GABARAPs and mice that lacked Gate-16 alone were defective in the IFN-gamma-induced clearance of vacuolar pathogens such as Toxoplasma. GABARAPs are uniquely required for antimicrobial host defense through cytosolic distribution of interferon-inducible GTPases. PMID: 28604719
  3. Development of LC3/GABARAP sensors containing a LIR and a hydrophobic domain to monitor autophagy. PMID: 28320742
  4. Ablation of GABARAP inhibits tumor initiation and progression through enhancement of both antitumor immunity and cell death signaling. PMID: 27124579
  5. Lipidation of the LC3/GABARAP family of autophagy proteins relies on a membrane-curvature-sensing domain in Atg3. PMID: 24747438
  6. These results support the regulatory role of Bcl-2 in autophagy and define GABARAP as a novel interaction partner involved in this intricate connection. PMID: 24240096
  7. Results indicate that, compared with LC3, GABARAP is enriched in the axonal initial segments (AIS). PMID: 23671684
  8. Gabarap functions in the immune system. It is involved in mitochondrial quality control in macrophages, and thus it influences Nlrp3 inflammasome-dependent inflammatory responses. PMID: 23427251
  9. ATG8-like proteins (MAP1LC3B, GABARAP and GABARAPL1) are novel interactors of MAPK15/ERK8, a MAP kinase involved in cell proliferation and transformation. PMID: 22948227
  10. GABARAP/p62 complex is responsible for impairment of glomerular function and that it retards recovery from the effects of doxorubicin. PMID: 22049402
  11. because of its stronger binding for hKOPR, GEC1 is able to be recruited by hKOPR sufficiently without membrane association via its C-terminal modification; however, du GABARAP appears to require C-terminal modifications to enhance KOPR expression. PMID: 21388957
  12. Results suggest that lysosomal turnover of GABARAP-PL is activated during the differentiation of C2C12 cells to myotubes without inactivation of the mTor kinase-signaling pathway. PMID: 16874098
  13. the organization of extrasynaptic GABA(A) receptors and GABARAP may provide a range of distinct inhibitory currents in the brain PMID: 16954214
  14. GABARAP binds to and promotes trafficking of the AT(1)R to the plasma membrane. PMID: 18497328

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Subcellular Location Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle.
Protein Families ATG8 family
Database Links

KEGG: mmu:56486

STRING: 10090.ENSMUSP00000018711

UniGene: Mm.272460

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