Recombinant Mouse Guanine nucleotide-binding protein subunit alpha-11 (Gna11)

Code CSB-YP009583MO
MSDS
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Source Yeast
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Code CSB-EP009583MO
MSDS
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Source E.coli
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Code CSB-EP009583MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009583MO
MSDS
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Source Baculovirus
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Code CSB-MP009583MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Gna11; Gna-11Guanine nucleotide-binding protein subunit alpha-11; G alpha-11; G-protein subunit alpha-11
Species
Mus musculus (Mouse)
Expression Region
1-359
Target Protein Sequence
MTLESMMACC LSDEVKESKR INAEIEKQLR RDKRDARREL KLLLLGTGES GKSTFIKQMR IIHGAGYSEE DKRGFTKLVY QNIFTAMQAM VRAMETLKIL YKYEQNKANA LLIREVDVEK VTTFEHQYVN AIKTLWSDPG VQECYDRRRE FQLSDSAKYY LTDVDRIATV GYLPTQQDVL RVRVPTTGII EYPFDLENII FRMVDVGGQR SERRKWIHCF ENVTSIMFLV ALSEYDQVLV ESDNENRMEE SKALFRTIIT YPWFQNSSVI LFLNKKDLLE DKILHSHLVD YFPEFDGPQR DAQAAREFIL KMFVDLNPDS DKIIYSHFTC ATDTENIRFV FAAVKDTILQ LNLKEYNLV
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Acts as an activator of phospholipase C. Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs).
Gene References into Functions
  1. Galpha11 is inhibitory to osteoanabolic actions of both parathyroid hormone and exercise PMID: 27300035
  2. results suggest that alveolar Gq/11 signaling maintains alveolar homeostasis and likely independently increases TGFbeta activation in response to the mechanical stress of the epithelium and decreases epithelial IL-33 synthesis. Together, these findings suggest that disruption of Gq/11 signaling promotes inflammatory emphysema but protects against mechanically induced lung injury. PMID: 27811142
  3. the results have identified 138 and 150 ovarian genes that are up-regulated or down-regulated, respectively, at the end of gestation in a Galphaq/11-dependent fashion. PMID: 26843449
  4. In Galpha11-knock-out mice there is a decreased heart rate and increased ejection fraction. PMID: 26476043
  5. ZIP9/Gna11 interactions mediate the non-classical testosterone signaling cascade in spermatogenic GC-2 cells. PMID: 26208885
  6. the conditional deletion of Galphaq/11 from granulosa/luteal cells prevents the progesterone withdrawal that occurs at the end of pregnancy and impairs parturition. PMID: 25495873
  7. Loss of gq/11 genes does not abolish melanopsin phototransduction. PMID: 24870805
  8. Mediated by lysophosphatidylinositol, Galpha(q/11) is activated in sensory neurons. PMID: 23973358
  9. Myocytes from the Galpha(q) and/or Galpha(11) KO mice displayed genuine alterations in the action potentials, membrane capacitance, membrane currents, and Ca(2+) handling PMID: 22562663
  10. Report differential expression of protein kinase C isoforms in coronary arteries of diabetic mice lacking Gna11. PMID: 21190563
  11. GPR54 regulates ERK1/2 activity and hypothalamic gene expression in a Galpha(q/11) and beta-arrestin-dependent manner. PMID: 20886089
  12. a signaling pathway via the G(q)-proteins, G(alpha)(11) and G(alpha)(q), that could link increased neurohumoral stimulation in diabetes with defective RyR2 channel function by regulating protein expression of FKBP12.6, annexin A7, and sorcin. PMID: 20372981
  13. Galpha(q) and Galpha(11) proteins serve as the intracellular mediators of endothelin-1 signaling in the pharyngeal arch mesenchyme PMID: 12648486
  14. Our findings suggest an expression level-dependent action of Galpha(q) and Galpha11 for Purkinje cell signaling and assign specific roles of these two G(q) isoforms for motor coordination PMID: 15175381
  15. G(q/11) signaling is indispensable to the neuronal circuit that connects the perception of pup-related stimuli to the initiation of maternal behavior PMID: 15340067
  16. G(q)/G(11)- but not G(12)/G(13)-mediated signaling processes mediate ET-1/ET(A)-dependent development of the cephalic neural crest. PMID: 15936338
  17. both Gq and G11 have critical roles in mediating inhibition of PTH secretion by extracellular Ca2+ PMID: 16988000
  18. lysophospholipids regulate integrin-mediated adhesion of splenic B cells to ICAM-1 through G(i) and G(12)/G(13) family G-proteins but not through G(q)/G(11) PMID: 17023430

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Subcellular Location
Cell membrane; Lipid-anchor. Cytoplasm.
Protein Families
G-alpha family, G(q) subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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