Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
Cd101; Igsf2; Immunoglobulin superfamily member 2; IgSF2; Glu-Trp-Ile EWI motif-containing protein 101; EWI-101; CD antigen CD101
Species
Mus musculus (Mouse)
Expression Region
22-266aa
Target Protein Sequence
REVKIQEGPLYRAEGYPVSIRCTVSGHQGPSTQDFRWSIYLPSAPTKEVQIISTKDAGFSYAVYAQRVQSKEIYIERLQGDSVLLHISKLQMKDAGEYECHTPNTDGKYFGSYSAKTNLTVVPDTLSATMPSQTLSKKEGEPLELTCETTKATVQHTHLSLTWYLMQEGGGSQATEIVSLSKDFVLTPGSSYADRFVAGDVRLDKLGATSFRLSVGKLQPSDQGQVFCEATEWIQDPDETWTLIT
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer，50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The gene responsible for the Mouse Cd101 protein (22-266aa) is cloned into a plasmid vector to obtain recombinant plasmid, which is then transformed into baculovirus cells. baculovirus cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to endure a specific antibiotic. The baculovirus cells containing the recombinant plasmid are cultured under conditions promoting the expression of the gene of interest. The protein carries a N-terminal 10xHis tag. Post-expression, affinity purification is employed to isolate and purify the recombinant Mouse Cd101 protein from the cell lysate. The resulting recombinant protein is subjected to denaturing SDS-PAGE, allowing for an estimation of its purity, surpassing 85%.
