Product Details

Purity

>85% (SDS-PAGE)

Target Names

Ipmk

Uniprot No.

Q7TT16

Alternative Names

Ipmk; Impk; Ipk2; Inositol polyphosphate multikinase; EC 2.7.1.140; EC 2.7.1.151; EC 2.7.1.153; Inositol 1,3,4,6-tetrakisphosphate 5-kinase

Species

Mus musculus (Mouse)

Expression Region

2-396

Target Protein Sequence

AAEPPALRL RPPGSTGDSP PVPRLLGGCV PLSHQVAGHM YGKDKVGILQ HPDGTVLKQL QPPPRGPREL EFYTMVYAAD CADAVLLELR KHLPKYYGVW SPPTAPNDVY LKLEDVTHKF NKPCIMDVKI GRKSYDPFAS SEKIQQQVSK YPLMEEIGFL VLGMRVYHLH SDSYETQNQH YGRGLTKETL KEGVSKFFHN GFCLRKDAIA ASIQKVEKIL QWFENQKQLN FYASSLLFVY EGSSQPATTK ANDRTLAGRF LSKGPLTDAD GLECNNNFHL FGAPPNGMSV GKSLSKAYSR HRKLYAKKHQ SQTSLKVETL EQDNGWRSMS QEHLNGNVLA QLEKVFYHLP AGRPEIPEAE VRMIDFAHVF PSNTVDEGYV YGLKHLIAVL RSILDS

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Inositol phosphate kinase with a broad substrate specificity. Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) first to inositol 1,3,4,5-tetrakisphosphate and then to inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5). Phosphorylates inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). Phosphorylates glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate to glycero-3-phospho-1D-myo-inositol 3,4,5-trisphosphate. Plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Binding of these highly phosphorylated inositol phosphates to MLKL mediates the release of an N-terminal auto-inhibitory region, leading to activation of the kinase. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis. Required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).

Gene References into Functions

these findings identify IPMK as a key determinant of TRAF6 stability and elucidate the physiological function of IPMK in TLR-induced innate immunity. PMID: 28439546
Data show that phospholipase C (PLC)-beta1 (PLC-beta1) overexpression determines an increase in beta-catenin translocation and that PLC-beta1, inositol polyphosphate multikinase (IPMK) and beta-catenin are mediators of the same signaling pathway. PMID: 27563828
a severe loss of IPMK in the striatum of Huntington disease patients and in several cellular and animal models of the disease, is reported. PMID: 26195796
metformin-mediated activation of AMP-Activated Protein Kinases was impaired in the absence of IPMK. PMID: 24877601
Inositol polyphosphate multikinase is a coactivator for serum response factor-dependent induction of immediate early genes. PMID: 24248338
Knockdown of expression and/or inhibition of function of phospholipase Cgamma1, inositol polyphosphate multikinase, which generates inositol 1,3,4,5-tetrakisphosphate (InsP) and InsP, and inositol hexakisphosphate kinase 1/2 PMID: 23322705
Inositol polyphosphate multikinase is a transcriptional coactivator required for immediate early gene induction. PMID: 24043835
IPMK regulates glucose signaling to AMPK in a pathway whereby glucose activates phosphorylation of IPMK at tyrosine 174 enabling the enzyme to bind to AMPK and regulate its activation. PMID: 22203993
Data show that in MEFs lacking IPMK, synthesis of IP5 and IP7 is abolished, but IP6 formation is reduced about 90%. PMID: 21220345
Inositol polyphosphate multikinase (Ipk2) plays a major role in the synthesis of inositol polyphosphate messengers derived from inositol 1,4,5-trisphosphate in embryogenesis and normal development. PMID: 15939867

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Subcellular Location

Nucleus.

Protein Families

Inositol phosphokinase (IPK) family

Database Links

KEGG: mmu:69718

STRING: 10090.ENSMUSP00000078240

UniGene: Mm.245867

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Recombinant Mouse Inositol polyphosphate multikinase (Ipmk)

Product Details

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Target Background

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