Recombinant Mouse Insulin-like growth factor 2 mRNA-binding protein 2 (Igf2bp2)

Code CSB-YP716282MO
MSDS
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Source Yeast
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Code CSB-EP716282MO
MSDS
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Source E.coli
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Code CSB-EP716282MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP716282MO
MSDS
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Source Baculovirus
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Code CSB-MP716282MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Igf2bp2
Uniprot No.
Alternative Names
Igf2bp2; Imp2; Vickz2Insulin-like growth factor 2 mRNA-binding protein 2; IGF2 mRNA-binding protein 2; IMP-2; IGF-II mRNA-binding protein 2; VICKZ family member 2
Species
Mus musculus (Mouse)
Expression Region
1-592
Target Protein Sequence
MMNKLYIGNL SPAVTADDLR QLFGDRKLPL AGQVLLKSGY AFVDYPDQNW AIRAIETLSG KVELHGKIME VDYSVSKKLR SRRIQIRNIP PHLQWEVLDG LLAEYGTVEN VEQVNTDTET AVVNVTYMTR EEAKLAIEKL SGHQFEDYSF KISYIPDEEV SSPSPPHRAR EQGHGPGSSS QARQIDFPLR ILVPTQFVGA IIGKEGLTIK NITKQTQSRV DIHRKENSGA AEKPVTIHAT PEGTSEACRM ILEIMQKEAD ETKLAEEVPL KILAHNGFVG RLIGKEGRNL KKIEHETGTK ITISSLQDLS IYNPERTITV RGTIEACANA EIEIMKKLRE AFENDMLAVN QQANLIPGLN LSALGIFSTG LSVLPPPAGP RGVPPSPPYH PFATHSGYFS SLYPHHHFGP FPHHHSYPEQ ETVSLFIPTQ AVGAIIGKKG AHIKQLARFA GASIKIAPAE GPDVSERMVI ITGPPEAQFK AQGRIFGKLK EENFFNPKEE VKLEAHIRVP SSTAGRVIGK GGKTVNELQN LTSAEVIVPR DQTPDENEEV IVRIIGHFFA SQTAQRKIRE IVQQVKQQEQ RYPQGVAPQR SK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts.
Gene References into Functions
  1. The IGF2 mRNA binding protein p62/IGF2BP2-2 induces fatty acid elongation as a critical feature of steatosis. PMID: 24755648
  2. results reveal a highly distinctive axonal enrichment of IMP2, show that it interacts with a network of axon guidance-related mRNAs, and reveal that it is required for normal axon pathfinding during vertebrate development. PMID: 27385015
  3. p62/IMP2 promotes hepatocarcinogenesis by an amplification of inflammation. PMID: 26426686
  4. IGF2BP2/IMP2-Deficient mice resist obesity through enhanced translation of Ucp1 mRNA and Other mRNAs encoding mitochondrial proteins. PMID: 25863250
  5. p62/IGF2BP2-2 drives the progression of NAFLD through elevation of hepatic iron deposition and increased production of hepatic free cholesterol. PMID: 25548482
  6. Mice with a liver overexpression of insulin-like growth factor 2 (IGF2) mRNA-binding protein p62/IMP2-2/IGF2BP2-2 show elevated Ccl2 levels, which models all stages of non-alcoholic fatty liver disease. PMID: 24173291
  7. data demonstrate that the HMGA2-IGF2BP2 axis functions as a key regulator of satellite cell activation and therefore skeletal muscle development PMID: 23177649
  8. oncofetal insulin-like growth factor 2 mRNA-binding protein 2 (IMP2, IGF2BP2) regulates oxidative phosphorylation (OXPHOS) in primary glioblastoma (GBM) sphere cultures (gliomaspheres) PMID: 22899010
  9. Dual phosphorylation of IMP2 at the mTOR sites is evident in the mouse embryo, likely coupling nutrient sufficiency to IGF2 expression and fetal growth PMID: 21576258

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
RRM IMP/VICKZ family
Tissue Specificity
Expressed in oocytes, granulosa cells of small and growing follicles and Leydig cells of the testis (at protein level). Expressed in testis and ovary.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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