Product Details

Purity

≥90% as determined by SDS-PAGE.

Activity

Not Test

Target Names

Il10ra

Uniprot No.

Q61727

Research Area

Immunology

Alternative Names

CDw210a;Interleukin-10 receptor subunit 1

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

17-241aa

Target Protein Sequence

LEFIAYGTELPSPSYVWFEARFFQHILHWKPIPNQSESTYYEVALKQYGNSTWNDIHICRKAQALSCDLTTFTLDLYHRSYGYRARVRAVDNSQYSNWTTTETRFTVDEVILTVDSVTLKAMDGIIYGTIHPPRPTITPAGDEYEQVFKDLRVYKISIRKFSELKNATKRVKQETFTLTVPIGVRKFCVKVLPRLESRINKAEWSEEQCLLITTEQYFTVTNLSI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

33.2 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.

Gene References into Functions

These results indicate that IL10R-mediated regulation of macrophage function during the early postnatal period is indispensable for preventing the development of murine colitis. PMID: 28678006
cross-talk between type I IFN and IL-10R signaling pathways may represent a key host cellular mechanism to investigate further in order to unravel the balance between pathogenic inflammation and homeostasis of the colon. Taken together, our data clearly demonstrate that type I IFN signaling is pathogenic in S. typhimurium colitis PMID: 29190678
this study shows that the upregulation of IL-10Ra by STAT3 contributed to the suppressive function of dendritic cellss following histone deacetylase inhibition PMID: 27866206
TR1 cell regulatory activity is dependent on IL-10 receptor signaling. PMID: 28003377
loss of IL-10R signaling impaired the generation and function of anti-inflammatory intestinal and bone-marrow-derived macrophages and their ability to secrete IL-10. PMID: 24792912
loss of IL-10 receptor expression impaired the critical conditioning of monocyte-derived macrophages and resulted in spontaneous development of severe colitis. PMID: 24792913
Recent findings review how IL10- and IL10R-dependent signaling modulates innate and adaptive immune responses in the murine intestine. PMID: 24507158
Treatment of mice with rIFN-gamma was sufficient to drive expression of IL-10R1 in the colonic epithelium. Intestinal epithelial-specific IL-10R1-null mice were more susceptible to DSS colitis associated with increased intestinal permeability. PMID: 24367025
Inhibition of IL-10R signaling during bacillus Calmette-Guerin (BCG) vaccination increases and balances IFN-gamma and IL-17A responses in favor of the host. PMID: 22972927
IL-10R subunit-alpha signaling plays a key role in the development as well as B-cell help function of T (follicular helper) cells in vitro and in vivo. PMID: 22753938
IL-10R blockade during chronic schistosomiasis mansoni results in the loss of B cells from the liver and the development of severe pulmonary disease. PMID: 22291593
showed here that IL-17A-producing CD4+ T cells expressed interleukin-10 receptor alpha (IL-10Ralpha) in vivo PMID: 21511184
reversal of tumor-induced dendritic cell paralysis by CpG immunostimulatory oligonucleotide and anti-interleukin 10 receptor antibody PMID: 12186845
Immunocytochemistry data shows that the region of the adrenal gland responsible for the vast majority of corticosterone synthesis, the zona fasciculata, predominantly expresses the IL-10 receptor 1. PMID: 16256304

Hide All

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Cytoplasm.

Protein Families

Type II cytokine receptor family

Database Links

KEGG: mmu:16154

STRING: 10090.ENSMUSP00000034594

UniGene: Mm.379327

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-EP720259MO
Abbreviation	Recombinant Mouse Il10ra protein, partial
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse Interleukin-10 receptor subunit alpha (Il10ra), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage