Recombinant Mouse LIM domain-containing protein 1 (Limd1)

Code CSB-YP865576MO
MSDS
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Source Yeast
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Code CSB-EP865576MO
MSDS
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Source E.coli
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Code CSB-EP865576MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865576MO
MSDS
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Source Baculovirus
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Code CSB-MP865576MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Limd1
Uniprot No.
Alternative Names
Limd1LIM domain-containing protein 1
Species
Mus musculus (Mouse)
Expression Region
1-668
Target Protein Sequence
MDKYDDLGLE ASKFIEDLNM YEASKDGLFR VDKGAGNNPE FEETRRVFAT KMAKIHLQQQ QQQQLLQEEA LPRAGRSPVN GGNRQGASGK LAADGAAKPP LAVPTVAPGL ATTTAAAQPS YPSQEQRIRP SAHGARPGSQ NCGSREGPVS SQRPALHGLS PSCEDPSCLT HGDYYDNFSL ASPQWGDKPE GCPSVSLGVG SGWPGCPGND STLPKSCGDH HPYQPQLSTV CSGRSFESGI SGQDGGIGGH SSEKPTGLWS TASSQRVNLG FSSMGLENGT SAQPKGTTVS APMVPSSASQ GACPKRDSGL GYEASGRVFK PLVDTQPWLQ DGPKSYLSVS APLSSTAGKD STQPGMTTGL DPKFGCVESG TSPKPSPTSN VHPVMSTPSE LSCKESSPSW STDSSLEPVL PGSPTPSRVR LPCQTLAPGP ELGPSTAELK LEALTQRLER EMDAHPKADY FGSCVKCSKG VFGAGQACQA MGDLYHDACF TCAACSRKLR GKAFYFVNGK VFCEEDFLYS GFQQSADRCF LCGHLIMDMI LQALGKSYHP GCFRCVICNE CLDGVPFTVD SENKIYCVRD YHKVLAPKCA ACGLPILPPE GSDETIRVVS MDRDYHVECY HCEDCGLELN DEDGHRCYPL EDHLFCHSCH VKRLEKGPSP APLHQHHF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation.
Gene References into Functions
  1. Limd1 influences not only stress osteoclastogenesis but also osteoblast function and osteoblast progenitor commitment. Together, these data identify Limd1 as a novel regulator of both bone osetoclast and bone osteoblast development and function. PMID: 18657804
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, P-body. Cell junction, adherens junction. Cell junction, focal adhesion.
Protein Families
Zyxin/ajuba family
Tissue Specificity
Ubiquitous.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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