Recombinant Mouse NADP-dependent malic enzyme (Me1)

Code CSB-YP013632MO
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Source Yeast
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Code CSB-EP013632MO
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Source E.coli
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Code CSB-EP013632MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013632MO
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Source Baculovirus
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Code CSB-MP013632MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Me1
Uniprot No.
Alternative Names
Me1; Mod-1; Mod1NADP-dependent malic enzyme; NADP-ME; EC 1.1.1.40; Malic enzyme 1
Species
Mus musculus (Mouse)
Expression Region
1-572
Target Protein Sequence
MEPRAPRRRH THQRGYLLTR DPHLNKDLAF TLEERQQLNI HGLLPPCIIS QELQVLRIIK NFERLNSDFD RYLLLMDLQD RNEKLFYSVL MSDVEKFMPI VYTPTVGLAC QQYSLAFRKP RGLFISIHDK GHIASVLNAW PEDVVKAIVV TDGERILGLG DLGCNGMGIP VGKLALYTAC GGVNPQQCLP ITLDVGTENE ELLKDPLYIG LRHRRVRGPE YDAFLDEFME AASSKYGMNC LIQFEDFANR NAFRLLNKYR NKYCTFNDDI QGTASVAVAG LLAALRITKN KLSDQTVLFQ GAGEAALGIA HLVVMAMEKE GLSKENARKK IWLVDSKGLI VKGRASLTEE KEVFAHEHEE MKNLEAIVQK IKPTALIGVA AIGGAFTEQI LKDMAAFNER PIIFALSNPT SKAECSAEQC YKVTKGRAIF ASGSPFDPVT LPDGRTLFPG QGNNSYVFPG VALGVVACGL RHIDDKVFLT TAEVISQQVS DKHLQEGRLY PPLNTIRGVS LKIAVKIVQD AYKEKMATVY PEPQNKEEFV SSQMYSTNYD QILPDCYPWP AEVQKIQTKV NQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. Data suggest that Ad4BP plays role in regulation of intracellular NADPH concentration via transcription of Me1 and Mthfd2 genes in adrenocortical cells. (Ad4BP = nuclear receptor subfamily 5 group A member 1; Me1 = malic enzyme 1; Mthfd2 = bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase) PMID: 28202838
  2. This study showed that (2)H-labeled tracers enable dissection of NADPH production routes across cell types and environmental conditions. PMID: 26999781
  3. ME1 expression was found to be mutant-KRAS-associated in an NSCLC mouse model. PMID: 26173780
  4. p53 represses the expression of the tricarboxylic-acid-cycle-associated malic enzymes ME1 and ME2 in human and mouse cells PMID: 23334421
  5. cytosolic or mitochondrial isoforms of malic enzyme do not affect glucose-stimulated insulin secretion from rodent islets PMID: 18755687
  6. although ME1 overexpression augments anaplerosis and glucose stimulated insulin secretion in INS-1 832/13 cells, it is not likely involved in methyl succinate and glucose stimulated insulin secretion in pancreatic islets PMID: 19293334

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Subcellular Location
Cytoplasm.
Protein Families
Malic enzymes family
Database Links
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