Recombinant Mouse Plasminogen activator inhibitor 2, macrophage (Serpinb2)

Code CSB-YP021070MO
MSDS
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Source Yeast
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Code CSB-EP021070MO
MSDS
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Source E.coli
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Code CSB-EP021070MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021070MO
MSDS
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Source Baculovirus
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Code CSB-MP021070MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Serpinb2
Uniprot No.
Alternative Names
Serpinb2; Pai2; Planh2Plasminogen activator inhibitor 2; macrophage; PAI-2; Serpin B2
Species
Mus musculus (Mouse)
Expression Region
1-415
Target Protein Sequence
MEELSMANTM FALNLLKQIE KSNSTQNIFI SPWSISSTLA IVLLGAGGNT EQQMAKVLQF NEIGSYGITT RNPENFSGCD FAQQIQKENY PSAILQAQAG DKIHSAFSSL SSTINTPQGD YLLESANKLF GEKSARFKEE YIQLSKKYYS TEPEAVDFLE CAEEAREKIN SWVKTQTKGE IPNLLPEGSV DEDTKMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSHESI PVQMMFLHAK LNIGYIKDLK TQILELPHTG NISMLLLLPD EIEDASTGLE LLESEINFAN FNKWISKDTL DEDDVVVYIP KFKLAQSYEL KSILQSMGME DAFNKGKANF SGMSERNDLF LSEVFHQASV DVTEEGTVAA GGTGAVMTGR TGHGGPQFVA DHPFLFFIMD KITHTILFVG RFSSP
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. Not required for normal murine development or survival.
Gene References into Functions
  1. This study establishes a novel role for SerpinB2 in the stromal compartment in pancreatic ductal adenocarcinoma invasion through regulation of stromal remodelling PMID: 28346421
  2. Data suggest that the function of keratinocyte SerpinB2 is protection of the stratum corneum from proteolysis via inhibition of urokinase, thereby maintaining the integrity and barrier function of the stratum corneum, particularly during times of skin inflammation PMID: 27109612
  3. SerpinB2 plays an important role in proteostas PMID: 26083412
  4. PAI-2 is a novel regulator of venous thrombus resolution, which modulates several pathways involving both inflammatory and uPA activity mechanisms, distinct from PAI-1 PMID: 25041188
  5. SerpinB2 has previously been implicated as a mediator of DMBA/TPA-induced skin carcinogenesis PMID: 25489104
  6. The presence of SerpinB2 on the surface of MPs provides a physiological mechanism whereby cancer cell SerpinB2 can reach the extracellular milieu and access urokinase plasminogen activator (uPA). PMID: 24644264
  7. Brain metastatic cells from lung cancer and breast cancer express high levels of anti-plasminogen activator (PA) serpins, including neuroserpin and serpin B2, to prevent plasmin generation and its metastasis-suppressive effects. PMID: 24581498
  8. C/EBP-beta mediates both constitutive and LPS-induced serpinB2 mRNA expression in mouse embryonic fibroblasts and inflammatory primary macrophages. PMID: 23472114
  9. Depletion PAI-2 resulted in NLRP3- and ASC-dependent caspase-1 activation and IL-1beta secretion in macrophages upon Toll-like receptor 2 (TLR2) and TLR4 engagement. PMID: 24043792
  10. SerpinB2 can be induced by lentiviral infection in vivo. PMID: 23460840
  11. immune regulation of SerpinB2 expression plays a critical role in the development of Th2-mediated protective immunity against nematode infection by a mechanism involving CCL2 production and macrophage infiltration. PMID: 23630350
  12. PAI-2 and TG2 are downstream mediators in the antiapoptotic response triggered upon TBK1 activation. PMID: 22203995
  13. Data show that regulation of Th1 responses thus appears to be a physiological function of inflammation-associated SerpinB2. PMID: 20130210
  14. during differentiation of epidermal keratinocyte, PAI-2 expresses mainly in the more differentiated keratinocytes and may protect the terminal differentiated keratinocytes from prematuration through inhibiting involucrin expression in cornified envelope PMID: 15050365
  15. PAI-2 promotes adipose tissue development in mice via a mechanism independent of its antifibrinolytic effect PMID: 17958744
  16. SerpinB2 is a cell survival factor that modulates Rb repression of proapoptotic signal transduction PMID: 18632617
  17. Plasminogen activator inhibitor-2, but not cystatin C, inhibits the prometastatic activity of tissue inhibitor of metalloproteinases-1 in the liver.( PMID: 18681831

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Subcellular Location
Cytoplasm. Secreted, extracellular space.
Protein Families
Serpin family, Ov-serpin subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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