Product Details

Purity

>85% (SDS-PAGE)

Target Names

Mycbp2

Uniprot No.

Q7TPH6

Alternative Names

Mycbp2; Pam; Phr1; E3 ubiquitin-protein ligase MYCBP2; EC 2.3.2.-; Myc-binding protein 2; Pam/highwire/rpm-1 protein; Protein Magellan; Protein associated with Myc

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues. Shows esterification activity towards both threonine and serine, with a preference for threonine, and acts via two essential catalytic cysteine residues that relay ubiquitin to its substrate via thioester intermediates. Interacts with the E2 enzymes UBE2D1, UBE2D3, UBE2E1 and UBE2L3. Plays a key role in neural development, probably by mediating ubiquitination of threonine residues on target proteins. Involved in different processes such as regulation of neurite outgrowth, synaptic growth, synaptogenesis and axon degeneration. Required for the formation of major central nervous system axon tracts. Required for proper axon growth by regulating axon navigation and axon branching: acts by regulating the subcellular location and stability of MAP3K12/DLK. Required for proper localization of retinogeniculate projections but not for eye-specific segregation. Regulates axon guidance in the olfactory system. Involved in Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons: acts by promoting destabilization of NMNAT2, probably via ubiquitination of NMNAT2. Catalyzes ubiquitination of threonine and/or serine residues on NMNAT2, consequences of threonine and/or serine ubiquitination are however unknown. Regulates the internalization of TRPV1 in peripheral sensory neurons. May mediate ubiquitination and subsequent proteasomal degradation of TSC2/tuberin. Independently of the E3 ubiquitin-protein ligase activity, also acts as a guanosine exchange factor (GEF) for RAN in neurons of dorsal root ganglia. May function as a facilitator or regulator of transcriptional activation by MYC. Acts in concert with HUWE1 to regulate the circadian clock gene expression by promoting the lithium-induced ubiquination and degradation of NR1D1.

Gene References into Functions

this study shows that Myc binding protein 2 suppresses M2-like phenotypes in macrophages during zymosan-induced inflammation in mice PMID: 29067676
nuclear localization of Ran was strongly increased in MYCBP2-deficient DRGs PMID: 26304119
Arf-bp1 and Pam are novel regulators of circadian gene expression that target Rev-erb alpha for degradation PMID: 20534529
Mycbp2 and Robo2 were found to cooperate within a genetic network that has profound effects on axon guidance PMID: 23525682
Phr1 is identified as a key regulator of a core axon degeneration program that balances axon survival and loss in various neuronal cell types. PMID: 23665224
The ubiquitin ligase complex containing Pam-Fbxo45 likely targets additional synaptic and axonal proteins, which may explain the overlapping neurodevelopmental defects observed in Phr1 and Fbxo45 deficiency. PMID: 22798074
These results show that Phr1 is an essential regulator of retinal ganglion cell projection during both dLGN and SC topographic map development. PMID: 21324225
p38 MAPK activation can inhibit activity-induced ion channel internalization and MYCBP2 regulates internalization of TRPV1 in peripheral sensory neurons as well as duration of thermal hyperalgesia through p38 MAPK PMID: 21098484
Structures of both the first and second PHR domains of Mus musculus (mouse) Phr1 (MYC binding protein 2, Mycbp2) have been determined, revealing a novel beta sandwich fold composed of 11 antiparallel beta-strands. PMID: 20156452
Phr1 is required for formation of major CNS axon tracts via a mechanism that is both cell-nonautonomous and independent of DLK. PMID: 17901218
Thus, efficacious pathfinding requires Phr1 activity for coordinating the cytoskeletal organization that distinguishes axons from growth cones. PMID: 18031680
Protein Associated with Myc (PAM) was identified as a binding partner of KCC2. PMID: 18769030
Results show that a loss-of-function allele of Phr1 resulting in respiratory distress at birth. PMID: 19391113

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Subcellular Location

Nucleus. Cell projection, axon. Cytoplasm, cytoskeleton.

Protein Families

Highwire family

Tissue Specificity

Expression is mostly restricted to the nervous system, including expression in motor and sensory axons. During postnatal development, expression is particularly strong in the cerebellum, hippocampus and retina. Lower levels of expression are observed thro

Database Links

KEGG: mmu:105689

STRING: 10090.ENSMUSP00000124710

UniGene: Mm.6478

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Recombinant Mouse Probable E3 ubiquitin-protein ligase MYCBP2 (Mycbp2), partial

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