Recombinant Mouse Protein HIRA (Hira), partial

Code CSB-YP717222MO
MSDS
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Source Yeast
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Code CSB-EP717222MO
MSDS
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Source E.coli
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Code CSB-EP717222MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP717222MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP717222MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hira
Uniprot No.
Alternative Names
Hira; Tuple1Protein HIRA; TUP1-like enhancer of split protein 1
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for the periodic repression of histone gene transcription during the cell cycle. Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Gene References into Functions
  1. These data suggest that elimination of HIRA produces a hypertrophic response in skeletal muscle and leaves myofibers susceptible to stress-induced degeneration. PMID: 28600325
  2. transcriptional regulation by HIRA is crucial for cardiomyocyte homeostasis. PMID: 26935106
  3. Data show that Hira is important to mediates Histone H3/H4 replacement during mouse oogenesis which is required for normal 5mC deposition in oocytes. Its loss results in chromatin abnormalities and extensive oocyte loss. PMID: 26549683
  4. Hira-mediated H3.3 incorporation is essential for parental genome reprogramming and reveal an unexpected role for rRNA transcription in the mouse zygote PMID: 25087892
  5. WHSC1 links transcription elongation to HIRA-mediated histone H3.3 deposition. PMID: 23921552
  6. HIRA is an essential factor for muscle development by establishing MyoD activation in myotubes. PMID: 21173268
  7. Targeted mutagenesis of the Hira gene results in gastrulation defects and patterning abnormalities of mesoendodermal derivatives prior to early embryonic lethality PMID: 11884616

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Subcellular Location
Nucleus. Nucleus, PML body.
Protein Families
WD repeat HIR1 family
Tissue Specificity
Expressed in cerebrum, cerebellum, heart, kidney, liver, lung and spleen.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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