Recombinant Mouse Protein spinster homolog 2 (Spns2), partial

Code CSB-YP849648MO
MSDS
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Source Yeast
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Code CSB-EP849648MO
MSDS
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Source E.coli
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Code CSB-EP849648MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP849648MO
MSDS
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Source Baculovirus
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Code CSB-MP849648MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Spns2
Uniprot No.
Alternative Names
Spns2; Protein spinster homolog 2
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts a a crucial lysosphingolipid sphingosine 1-phosphate (S1P) transporter involved in S1P secretion and function. S1P is a bioactive signaling molecule that regulates many physiological processes important for the development and for the immune system. Regulates levels of S1P and the S1P gradient that exists between the high circulating concentrations of S1P and low tissue levels that control lymphocyte trafficking.
Gene References into Functions
  1. genome-wide in vivo screen identifies novel host regulators of metastatic colonization, such as Spns2 PMID: 28052056
  2. results point to a broad role for Spns2-mediated S1P transport in the initiation and development of adaptive immune related disorders PMID: 26324848
  3. Spns2 expression by endothelial cells is essential for secretion of lymph S1P, while Spns2 is dispensable for RBC secretion of plasma S1P. PMID: 23103166
  4. Spns2 is an S1P transporter in vivo that plays a role in regulation not only of blood S1P but also lymph node and lymph S1P levels and consequently influences lymphocyte trafficking and lymphatic vessel network organization. PMID: 23180825
  5. SPNS2 is the first physiological S1P transporter in mammals and is a key determinant of lymphocyte egress from the thymus. PMID: 22723910
  6. The changes in lymphocyte subpopulations in mutant Spns2 mice closely mimick the phenotypes of partial sphingosine-1-phosphate (S1P) deficiency, impaired S1P-dependent lymphocyte trafficking, and depletion of circulating lymphocytes. PMID: 22664872
  7. Spns2 functions in endothelial cells to establish the S1P gradient required for T and B cells to egress from their respective primary lymphoid organs. PMID: 22406534
  8. The sphingosine 1-phosphate transporter, SPNS2, functions as a transporter of the phosphorylated form of the immunomodulating agent FTY720. PMID: 21084291

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein.
Protein Families
Major facilitator superfamily, Spinster (TC 2.A.1.49) family
Tissue Specificity
Expression is high in the lungs and liver, low in the lymph nodes, spleen and bone marrow, and very low but detectable in the thymus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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