Recombinant Mouse Purkinje cell protein 4 (Pcp4)

Code CSB-YP017636MO
MSDS
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Source Yeast
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Code CSB-EP017636MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017636MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP017636MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pcp4
Uniprot No.
Alternative Names
Pcp4; Pep19; Calmodulin regulator protein PCP4; Brain-specific antigen PCP-4; Brain-specific polypeptide PEP-19; Purkinje cell protein 4
Species
Mus musculus (Mouse)
Expression Region
1-62
Target Protein Sequence
MSERQSAGAT NGKDKTSGDN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as a modulator of calcium-binding by calmodulin. Thereby, regulates calmodulin activity and the different processes it controls. For instance, may play a role in neuronal differentiation through activation of calmodulin-dependent kinase signaling pathways.
Gene References into Functions
  1. PEP-19/pcp4 has previously been identified as a critical determinant of synaptic plasticity. PMID: 27760390
  2. We further found that Pcp4 gene on the Ts1Rhr trisomic segment is expressed in ependymal cells, and its resumption to two copies rescued both ventricular enlargement and cilia dysfunction in Ts1Rhr mice. PMID: 28069794
  3. PCP4 regulates cardiac excitability through both Purkinje cell-autonomous and central mechanisms. PMID: 25295538
  4. The PCP4 immunostaining-delineated CA2 neurons have distinguishable differences in cell morphology, physiology, and synaptic circuit connections compared with distal CA3 and proximal CA1 regions. PMID: 24166578
  5. Data show that the transcription factor Hoxa2 binds to Pcp4 chromatin and regulates Pcp4 expression in the second arch. PMID: 23671666
  6. Data show that Pcp4 overexpression induces precocious neuronal differentiation, and is associated with an increase in CaMKIIdelta activation. PMID: 21491429
  7. Data indicate that pep-19/pcp4 is a critical determinant of synaptic plasticity in cerebellum and locomotor learning. PMID: 21576365
  8. The expression of Pcp4 in naive resting mature B cells is dependent upon tonic stimulation from the CR1/CR2 proteins via a C3 ligand. PMID: 19201479

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Protein Families
PCP4 family
Database Links
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