Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Target Names

Stac3

Uniprot No.

Q8BZ71

Research Area

Cell Biology

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-360aa

Target Protein Sequence

MTEKEVVESPQPPFPGETPQSGLQRLKQLFKKGSPETAEMEPPPEPQANGEAVGAGGGPIYYIYEEEEEEEEEEEPPPEPPKLVNDKPHKFKDHFFKKPKFCDVCARMIVLNNKFGLRCKNCKTNIHEHCQSYVEMQRCFGKIPPGFRRAYSSPLYSDQQYAVSAANRNDPVFETLRVGVIMANKERKKGQADKKNPLAAMMEEEPESARPEEGKSQDGNNAEKDKKAEKKTPDDKNKQPGFQQSHYFVALYRFKALEKDDLDFPPGEKITVIDDSNEEWWRGKIGEKVGFFPPNFIIRVRAGERVHRVTRSFVGNREIGQITLKKDQIVVQKGDEAGGYVKVYTGRKVGLFPTDFLEEI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

47.9 kDa

Protein Length

Full Length

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Required for normal excitation-contraction coupling in skeletal muscle and for normal muscle contraction in response to membrane depolarization. Required for normal Ca(2+) release from the sarcplasmic reticulum, which ultimately leads to muscle contraction. Probably functions via its effects on muscle calcium channels. Increases CACNA1S channel activity, in addition to its role in enhancing the expression of CACNA1S at the cell membrane. Has a redundant role in promoting the expression of the calcium channel CACNA1S at the cell membrane. Slows down the inactivation rate of the calcium channel CACNA1C.

Gene References into Functions

Thus, the C1 domain of STAC3 is responsible for its stable incorporation into the voltage-sensor (CaV1.1) of excitation-contraction-coupling complex, whereas the SH3 domain containing the Native American myopathy mutation site may be involved in low-affinity functional interactions in excitation-contraction-coupling. PMID: 28112192
These data together confirm a critical role of STAC3 in excitation-contraction coupling but also suggest that STAC3 may have additional functions in skeletal muscle, at least in the hindlimb muscle. PMID: 27184118
Stac3 is directly involved in conformational coupling between CaV1.1 and RyR1. PMID: 27621462
STAC3 is important to myofiber hypertrophy, myofiber-type composition, contraction, and excitation-induced calcium release from the sarcoplasmic reticulum in the postnatal skeletal muscle. PMID: 27073615
Stac3 may play a role in preventing precocious myoblast differentiation during skeletal muscle development PMID: 24788338
Stac3 gene plays a critical role in skeletal muscle development and function in mice. PMID: 23626854
STAC3 mediates voltage-induced Ca2+ release and contractility in skeletal muscles. PMID: 23818578

Hide All

Subcellular Location

Cytoplasm. Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Cell membrane, sarcolemma, T-tubule.

Tissue Specificity

Dected in skeletal muscle, including soleus, extensor digitorum longus, tibialis anterior, quadriceps and gastrocnemius. Detected in tongue.

Database Links

KEGG: mmu:237611

STRING: 10090.ENSMUSP00000048148

UniGene: Mm.32087

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-EP805368MO
Abbreviation	Recombinant Mouse Stac3 protein
MSDS	MSDS Datasheet
Size	$256
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse SH3 and cysteine-rich domain-containing protein 3 (Stac3)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage