Recombinant Mouse SH3 and cysteine-rich domain-containing protein 3 (Stac3)

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Stac3

Uniprot No.

Q8BZ71

Alternative Names

Stac3; SH3 and cysteine-rich domain-containing protein 3

Species

Mus musculus (Mouse)

Expression Region

1-360

Target Protein Sequence

MTEKEVVESP QPPFPGETPQ SGLQRLKQLF KKGSPETAEM EPPPEPQANG EAVGAGGGPI YYIYEEEEEE EEEEEPPPEP PKLVNDKPHK FKDHFFKKPK FCDVCARMIV LNNKFGLRCK NCKTNIHEHC QSYVEMQRCF GKIPPGFRRA YSSPLYSDQQ YAVSAANRND PVFETLRVGV IMANKERKKG QADKKNPLAA MMEEEPESAR PEEGKSQDGN NAEKDKKAEK KTPDDKNKQP GFQQSHYFVA LYRFKALEKD DLDFPPGEKI TVIDDSNEEW WRGKIGEKVG FFPPNFIIRV RAGERVHRVT RSFVGNREIG QITLKKDQIV VQKGDEAGGY VKVYTGRKVG LFPTDFLEEI

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Related Products

Target Background

Function

Required for normal excitation-contraction coupling in skeletal muscle and for normal muscle contraction in response to membrane depolarization. Required for normal Ca(2+) release from the sarcplasmic reticulum, which ultimately leads to muscle contraction. Probably functions via its effects on muscle calcium channels. Increases CACNA1S channel activity, in addition to its role in enhancing the expression of CACNA1S at the cell membrane. Has a redundant role in promoting the expression of the calcium channel CACNA1S at the cell membrane. Slows down the inactivation rate of the calcium channel CACNA1C.

Gene References into Functions

Thus, the C1 domain of STAC3 is responsible for its stable incorporation into the voltage-sensor (CaV1.1) of excitation-contraction-coupling complex, whereas the SH3 domain containing the Native American myopathy mutation site may be involved in low-affinity functional interactions in excitation-contraction-coupling. PMID: 28112192
These data together confirm a critical role of STAC3 in excitation-contraction coupling but also suggest that STAC3 may have additional functions in skeletal muscle, at least in the hindlimb muscle. PMID: 27184118
Stac3 is directly involved in conformational coupling between CaV1.1 and RyR1. PMID: 27621462
STAC3 is important to myofiber hypertrophy, myofiber-type composition, contraction, and excitation-induced calcium release from the sarcoplasmic reticulum in the postnatal skeletal muscle. PMID: 27073615
Stac3 may play a role in preventing precocious myoblast differentiation during skeletal muscle development PMID: 24788338
Stac3 gene plays a critical role in skeletal muscle development and function in mice. PMID: 23626854
STAC3 mediates voltage-induced Ca2+ release and contractility in skeletal muscles. PMID: 23818578

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Subcellular Location

Cytoplasm. Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Cell membrane, sarcolemma, T-tubule.

Tissue Specificity

Dected in skeletal muscle, including soleus, extensor digitorum longus, tibialis anterior, quadriceps and gastrocnemius. Detected in tongue.

Database Links

KEGG: mmu:237611

STRING: 10090.ENSMUSP00000048148

UniGene: Mm.32087

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Recombinant Mouse SH3 and cysteine-rich domain-containing protein 3 (Stac3)

Product Details

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Target Background

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