Recombinant Mouse Semaphorin-3G (Sema3g)

Code CSB-EP705975MO
MSDS
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Target Names
Sema3g
Uniprot No.
Research Area
Immunology
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
23-780aa
Target Protein Sequence
SPGPSPSVPRLRLSYRDLLSTNRSAIFLGPRGSLDLQVMYLDEYRDRLFLGSRDALYSLRLDQAWPDPREVLWLPQPGQKVECVRKGKDPLTECANFVRVLQPHNRTHLLACGTGAFQPICTFITVGHRGEHVLRLDASSVENGRGRCPHEPSRPFASTFVGGELYTGLTADFLGREAMIFRSGGPRPALRSDSDQSLLHEPRFVMAARIPDNSDRDDDKVYFFFSETVPSPDGGPGHVTISRVGRVCVNDAGGQRVLVNKWSTFLKARLVCSVPGPGGAETHFDQLEDVFLLWPKAGKSLEVYALFSTVSAVFQGFAVCVYHMVDIWEVFNGPFAHRDGPQHQWGPYGGKVPFPRPGVCPSKMTAQPGRPFGSTKDYPDEVLQFVRDHPLMFQPVRPRRGRPVLVKTHLAQRLRQIVVDRVEAEDGTYDVIFLGTDSGSVLKVIALQGGGLTEPEEVVLEELQVFKVPTPITEMEISVKRQTLYVGSPLGVARLQLHQCETYGSACAECCLARDPYCAWDGTACARYRPSSGKRRFRRQDIRHGNPAVQCLGQGQSQNKAASGLMTRVFGTEHNSTFLECLPKSPQAAVRWFLQRPGDKGTDQVKTDERVVQTAQGLLFRRLSRHDAGNYTCTTLEHGFSQTVVRFALEVIAAVQLDSLFLRESRLEEPSAWGSLASASPKTWYKDILQLTGFANLPRVDEYCERVWCRGVGERSGSFRGKGKQAKGKSWAGLELGKKMKSRVLAEHNRTPREVEAT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
91.3 kDa
Protein Length
Full Length of Mature Protein
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Has chemorepulsive activities for sympathetic axons. Ligand of NRP2.
Gene References into Functions
  1. Results indicate that Sema3G protein is secreted by podocytes and protects them from inflammatory kidney diseases and diabetic nephropathy. PMID: 27180624
  2. Sema3G induces cell collapse in an Nrp2/PlexinD1-dependent manner. PMID: 27880905
  3. Identify Sema3G as a primarily endothelial cell-expressed class 3 semaphorin that controls endothelial and smooth muscle cell functions in autocrine and paracrine manners. PMID: 20947821
  4. In adulthood, Sema3G is mainly expressed in the lung and kidney, and a little in the brain. We also found that Sema3G binds Neuropilin-2, but not Neuropilin-1, and induces the repulsion of sympathetic axons. PMID: 16098142
Subcellular Location
Secreted.
Protein Families
Semaphorin family
Tissue Specificity
Highly expressed in lung and kidney. Weakly expressed in brain.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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