Recombinant Mouse Semaphorin-4A (Sema4a), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Sema4a

Uniprot No.

Q62178

Alternative Names

Sema4a; Semab; SemB; Semaphorin-4A; Semaphorin-B; Sema B

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. Regulates glutamatergic and GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner and promotes the development of excitatory synapses in a PLXNB2-dependent manner. Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. Promotes phosphorylation of TIMD2. Inhibits angiogenesis. Promotes axon growth cone collapse. Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

Gene References into Functions

Suggest a role for Plexin-B1 as a ligand and Sema4A as a receptor and characterize a reverse signaling pathway downstream of Sema4A regulating cell migration via Scrib. PMID: 28007914
SEMA4A is required for optimal activation of mTORC1 in CD8+ T cells. PMID: 26116513
Treg-cell stability can be subverted in certain inflammatory sites, but is maintained by a Sema4a-Nrp1 axis-- this pathway is a potential therapeutic target that could limit Treg-cell-mediated tumour-induced tolerance without inducing autoimmunity PMID: 23913274
Data indicate that both semaphorin 4A mRNA and protein were clearly detected on the earliest progenitors and were downregulated through thymic development. PMID: 23648820
These results indicate the importance of the Sema4A protein conformation in human and mouse retina homeostasis. PMID: 23360997
A fraction of Sema4a-deficient transgenic BALB/c mice spontaneously develop skin lesions resembling atopic dermatitis (AD) in humans. PMID: 23007237
These data provide a new insight into Sema4A biology and define Sema4A as an important regulator of Th2-driven lung pathophysiology. PMID: 22472774
Sema4A activates a specialized and restricted genetic program in macrophages able to sustain angiogenesis and participates in their recruitment and activation in inflammatory injuries. PMID: 22442441
Sema4A regulates two distinct endosomal-sorting pathways that are critical for photoreceptor survival and phototransduction during the transition between daylight and darkness. PMID: 22465952
experiments using knockout mice revealed that Sema4A but not Sema7A was required for the effect produced by Th1 cells on neurite outgrowth from cortical neurons. PMID: 20946887
Data indicated that Sema4A could function as a chemo-repulsive cue by activating a receptor whose signal is transmitted to Rho-kinase and induced growth cone collapse of hippocampal neurons. PMID: 15942687
These results indicated that Sema4A is critically involved in experimental autoimmune myocarditis by regulating differentiation of T-cells. PMID: 18977352

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Semaphorin family

Tissue Specificity

Expressed in neurons and glia in the developing hippocampus.

Database Links

KEGG: mmu:20351

STRING: 10090.ENSMUSP00000029700

UniGene: Mm.439752

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Recombinant Mouse Semaphorin-4A (Sema4a), partial

Product Details

Customer Reviews and Q&A

Target Background

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