Product Details

Purity

>85% (SDS-PAGE)

Target Names

Pak3

Uniprot No.

Q61036

Alternative Names

Pak3; Pak-3; Pakb; Stk4; Serine/threonine-protein kinase PAK 3; EC 2.7.11.1; Beta-PAK; CDC42/RAC effector kinase PAK-B; p21-activated kinase 3; PAK-3

Species

Mus musculus (Mouse)

Expression Region

1-559

Target Protein Sequence

MSDSLDNEEK PPAPPLRMNS NNRDSSALNH SSKPLPMAPE EKNKKARLRS IFPGGGDKTN KKKEKERPEI SLPSDFEHTI HVGFDAVTGE FTPDLYGSQM CPGKLPEGIP EQWARLLQTS NITKLEQKKN PQAVLDVLKF YDSKETVNNQ KYMSFTSGDK SAHGYIAAHQ SNTKTASEPP LAPPVSEEED EEEEEEEDDN EPPPVIAPRP EHTKSIYTRS VVESIASPAA PNKEDIPPSA ENANSTTLYR NTDRQRKKSK MTDEEILEKL RSIVSVGDPK KKYTRFEKIG QGASGTVYTA LDIATGQEVA IKQMNLQQQP KKELIINEIL VMRENKNPNI VNYLDSYLVG DELWVVMEYL AGGSLTDVVT ETCMDEGQIA AVCRECLQAL DFLHSNQVIH RDIKSDNILL GMDGSVKLTD FGFCAQITPE QSKRSTMVGT PYWMAPEVVT RKAYGPKVDI WSLGIMAIEM VEGEPPYLNE NPLRALYLIA TNGTPELQNP ERLSAVFRDF LNRCLEMDVD RRGSAKELLQ HPFLKLAKPL SSLTPLIIAA KEAIKNSSR

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC).

Gene References into Functions

Pak3 knockout (KO) mice had defects of oligodendrocyte differentiation in developing corpus callosum and to a lesser extent in anterior commissure, which were compensated at the adult stage. In vitro experiments in oligodendrocyte precursor (OPC) cultures, derived from Pak3 KO and wild type brains, support a developmental and cell-autonomous role for PAK3 in regulating OPC differentiation into mature oligodendrocytes. PMID: 27940202
Our results indicate that Pak3 is a novel regulator of beta-cell differentiation and function. Pak3 acts downstream of Ngn3 to promote cell cycle exit and differentiation in the embryo by a mechanism that might involve repression of Ccnd1. PMID: 24163148
PAK3 regulates activity-mediated rearrangement of synaptic connectivity associated with learning suggesting that defects in spine formation and refinement during development could account for intellectual disability. PMID: 22238087
potential role of PAK3 in the progression of ACTH-producing thymic carcinoid PMID: 20960100
determination of modified specificities toward Rac and Cdc42 GTPase PMID: 12464619
This study results provide evidence for critical relationships between synaptic morphology and bidirectional modifiability of synaptic strength in the cortex and consolidation of long-term memory. PMID: 15182717
PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PMID: 15574732
A dramatic reduction in the active form of transcription factor cAMP-responsive element-binding protein in Pak3 knock-out mice implicates a novel signaling mechanism by which PAK3 and Rho signaling regulate synaptic function and cognition. PMID: 16014725
PAK3 regulates dendritic spines morphogenesis. PMID: 17105769
A new PAK3 alternatively spliced exon called c is located upstream of previously identified exon b. Exon b is detected in all tetrapods; exon c is only present in mammals. PMID: 18507705
Regulation and role of p21-activated kinase 3 by corticotropin-releasing factor in mouse pituitary PMID: 18940205

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Subcellular Location

Cytoplasm.

Protein Families

Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily

Database Links

KEGG: mmu:18481

STRING: 10090.ENSMUSP00000033640

UniGene: Mm.40035

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Recombinant Mouse Serine/threonine-protein kinase PAK 3 (Pak3)

Product Details

Customer Reviews and Q&A

Target Background

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