Recombinant Mouse Serine/threonine-protein phosphatase 6 catalytic subunit (Ppp6c)

Code CSB-YP863425MO
MSDS
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Source Yeast
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Code CSB-EP863425MO
MSDS
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Source E.coli
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Code CSB-EP863425MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863425MO
MSDS
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Source Baculovirus
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Code CSB-MP863425MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ppp6c
Uniprot No.
Alternative Names
Ppp6c; Serine/threonine-protein phosphatase 6 catalytic subunit; PP6C; EC 3.1.3.16
Species
Mus musculus (Mouse)
Expression Region
1-305
Target Protein Sequence
MAPLDLDKYV EIARQCKYLP ENDLKRLCDY VCDLLLEESN VQPVSTPVTV CGDIHGQFYD LCELFRTGGQ VPDTNYIFMG DFVDRGYYSL ETFTYLLALK AKWPDRITLL RGNHESRQIT QVYGFYDECQ TKYGNANAWR YCTKVFDMLT VAALIDEQIL CVHGGLSPDI KTLDQIRTIE RNQEIPHKGA FCDLVWSDPE DVDTWAISPR GAGWLFGAKV TNEFVHINNL KLICRAHQLV HEGYKFMFDE KLVTVWSAPN YCYRCGNIAS IMVFKDVNTR EPKLFRAVPD SERVIPPRTT TPYFL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalytic subunit of protein phosphatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. During mitosis, regulates spindle positioning. Downregulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7. Participates also in the innate immune defense against viruses by desphosphorylating RIG-I/DDX58, an essential step that triggers RIG-I/DDX58-mediated signaling activation.
Gene References into Functions
  1. Ppp6c deficiency enhances K-ras(G12D) -dependent tumor promotion PMID: 29758119
  2. the Ppp6c phosphatase domain is indispensable for mouse embryogenesis after implantation. PMID: 26868000
  3. findings uncover a hitherto unknown role for PP6 as an indispensable regulator of oocyte meiosis and female fertility. PMID: 26349807
  4. Data identify NF-kappaB-induced miR-31 and its target, ppp6c, as critical factors for the hyperproliferation of epidermis in psoriasis. PMID: 26138368
  5. Ppp6c deficiency predisposes mice to skin carcinogenesis initiated by DMBA. PMID: 25486434
  6. Ppp6c deficiency underlies molecular events that drive outgrowth of initiated keratinocytes harboring UVB-induced mutated p53 PMID: 26054846
  7. Our studies demonstrate that PP6 acts as a critical negative regulator of naive T cell homeostasis PMID: 25609840
  8. PP6 regulates the sensitivity of GBM cells to radiation, and suggest small molecules disrupting or inhibiting PP6 association with DNA-PK is a potential radiosensitizer for GBM. PMID: 22158480
  9. alpha4 expression differentially increased or decreased phosphorylation of endogenous phosphoproteins, consistent with opposing effects on PP2A and PP6 [PP6] PMID: 16895907

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Subcellular Location
Mitochondrion. Cytoplasm.
Protein Families
PPP phosphatase family, PP-6 (PP-V) subfamily
Tissue Specificity
Ubiquitously expressed in all tissues tested with strongest expression in lung, spleen, liver, kidney and brain. Weaker expression observed in bladder, pancreas, heart and skeletal muscle.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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