Recombinant Mouse Sodium/hydrogen exchanger 1(Slc9a1) ,partial

Code CSB-YP717200MO
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Source Yeast
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Code CSB-EP717200MO
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Source E.coli
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Code CSB-EP717200MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP717200MO
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Source Baculovirus
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Code CSB-MP717200MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Slc9a1
Uniprot No. Q61165
Alternative Names Slc9a1; Nhe1; Sodium/hydrogen exchanger 1; Na(+)/H(+) exchanger 1; NHE-1; Solute carrier family 9 member 1
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Gene References into Functions
  1. The results demonstrate that in renal cells, NHE1 is associated with several regulatory proteins including Hsp90, and that Hsp90 affects its function possibly through altered phosphorylation of the protein via the AKT kinase. PMID: 28483634
  2. This study showed that extracellular acidification inhibits NBCn1 and NHE1 activity in VSMCs; NBCn1 is equivalently inhibited when pCO2 is raised or the bicarbonate level decreased. Lowering the sodium level inhibits NBCn1 and NHE1 markedly only below the typical physiological and pathophysiological range. PMID: 28319329
  3. Cell volume homeostasis requiring Na+/H+ exchange signaled by JAK2 first becomes prominent during mouse embryonic development at the late one-cell stage. PMID: 28339658
  4. Lack of Dicer leads to oxidative stress, cytosolic acidification, upregulated NHE1 expression and activity as well as swelling of CD4+ T cells, functions all reversed by miR-15b or miR-200b. PMID: 28704808
  5. DJ-1 is a powerful regulator of reactive oxygen species production as well as NHE1 expression and activity in CD4(+) T cells. PMID: 27509531
  6. Overexpressed NHE1 suppresses the expression of ABCA1 protein via increasing the calpain activity in RAW264.7 cells. PMID: 28031107
  7. Inhibition of NHE1 by siRNA-NHE1 or with cariporide in CD4(+) T helper 9 (Th9) cells down-regulated IL-9 and ATP production. PMID: 27629415
  8. The current study concluded that NHE1 activity and pHi homeostasis are regulated by CoCl2 treatment in a time-dependent manner in astrocytes, and may be responsible for the changes in cell viability and injury observed under hypoxia-mimetic conditions induced by CoCl2 treatment. PMID: 27035646
  9. During hypoxia, activation of ROCK enhances NHE1 activity and promotes pulmonary artery smooth muscle cell migration and proliferation. PMID: 27009277
  10. Na/H exchanger is regulated in dendritic cells by Akt1. PMID: 26138884
  11. our results suggest that activation of NHE1 induces hypertrophy through the activation of NFAT3/Gata4 and OPN expression PMID: 25758355
  12. NHE1 function is necessary for maintaining mammary branched architecture. PMID: 24038847
  13. the extracellular matrix (ECM) composition strongly impacts on NHE1-dependent in vitro cell motility and invasion PMID: 24173371
  14. long-term ablation of NHE1 activity increases the redox potential, mitigates high-fat diet-induced myocardial stress. PMID: 24080184
  15. study shows that NHE-1 protein is abundantly expressed in microglial lamellipodia and maintains alkaline pHi in response to BK stimulation PMID: 23991215
  16. exerts a protective effect by reducing the loss of synaptopodin in podocytes exposed to endoplasmic reticulum stress PMID: 24566932
  17. NHE1 functions as a metabolic sensor for lipotoxicity. PMID: 24531551
  18. It plays a role in the control of cell volume and cintracellular pH. PMID: 23800993
  19. PDGFRalpha signaling in the primary cilium regulates NHE1-dependent fibroblast migration via coordinated differential activity of MEK1/2-ERK1/2-p90RSK and AKT signaling pathways. PMID: 23264740
  20. Role in growth factor-induced mammary branching morphogenesis. PMID: 22366186
  21. Fibronectin stimulates migration through lipid raft dependent NHE-1 activation in mouse embryonic stem cells. PMID: 22683701
  22. It was shown that preimplantation embryos possess an inorganic ion-dependent mechanism for acute cell volume control that relies on Na+ uptake mediated by NHE1 activation via Jak2 signaling and coupled Cl- uptake via HCO3-/Cl- exchange. PMID: 22740348
  23. Extracellular acidosis induces Ca(2+) oscillation via NHE1, leading to Ca(2+)/CaMKII-dependent CREB activation in human central nervous system pericytes. PMID: 22922957
  24. gender-specific activation of NHE1 may be critical and that NHE1 plays a more critical role in promoting some types of hypertrophy in females in comparison with males. PMID: 22627677
  25. found that NHE-1 is preferentially located in cholesterol-rich microdomains and that B[a]P activates NHE-1 via its relocation and binding of calmodulin outside these specialized plasma membrane microstructures PMID: 22613966
  26. Blood pressure was 25 mmHg lower in NHE1 knockout than wild-type mice. PMID: 22351634
  27. NHE1 directly binds to calcineurin A and activates downstream NFAT signaling, leading to cardiomyocyte hypertrophy PMID: 22688515
  28. Na+/H+ exchanger plays a key role in enhancing cytoplasmic Ca(2+) overload during ischemia-reperfusion and severely impairing post-ischemic cardiac function in hearts from type 2 diabetic db/db mice. PMID: 22490613
  29. Azathioprine interferes with the activation of dendritic cell Na(+)/H(+) exchanger by bacterial lipopolysaccharides, an effect likely participating in the anti-inflammatory action of the drug. PMID: 22508060
  30. In bone marrow derived dendritic cells, rapamycin blunts or virtually abrogates the stimulation of Na+/H+ exchanger activity, cell swelling and TNF-alpha release by lipopolysacharides. PMID: 22508061
  31. Phosphoinositide binding differentially regulates NHE1 Na+/H+ exchanger-dependent proximal tubule cell survival PMID: 22020933
  32. functional NHE2, but not NHE1, is essential for mouse gastric epithelial restitution and TFFs activate epithelial repair via NHE2 PMID: 21900251
  33. expression of activated NHE1 promotes greater sensitivity to neurohormonal stimulation PMID: 21359875
  34. Inhibiting the Na+/H+ exchanger reduces reperfusion injury. PMID: 21196287
  35. Expression of activated NHE1 elicits specific pathways of gene activation in the myocardium that lead to cardiac hypertrophy, cell death and infarction. PMID: 20460605
  36. The study suggests that NHE-1 functions to maintain microglial pH(i) homeostasis allowing for sustained NADPH oxidase function and "respiratory" burst. PMID: 21068326
  37. Nedd4-1 and beta-arrestin-1 as key regulators of NHE1 ubiquitylation, endocytosis, and function. PMID: 20855896
  38. Reperfusion triggers a transient stimulation of ribosomal protein S6 kinase 90kD 1 (p90RSK) pathway which contributes to cerebral ischemic damage in part via phosphorylation of NHE-1 protein. PMID: 20557427
  39. thyroid hormone and thyroid hormone receptor TRalpha regulate expression of the Na+/H+ exchanger PMID: 12039959
  40. NHE1 has a role in the generation of acidic extracellular domains of the lower SC, thus providing the acidification of deep SC interstices necessary for lipid processing and barrier homeostasis PMID: 12221084
  41. NHE1 activity is critical for Renal tubular epithelial cell survival after injury; caspase cleavage of RTC NHE1 may promote apoptosis and tubular atrophy by preventing compensatory intracellular volume and pH regulation PMID: 12453872
  42. NHE1 has a role in establishing polarity in migrating fibroblasts PMID: 12486114
  43. the mRNAs of different NHE isoforms as well as their subcellular localization (apical versus basolateral) and functional activity were investigated in mouse endometrial epithelial cells PMID: 12493726
  44. mice lacking NHE1 upregulate their Na(+) channel expression in the hippocampal and cortical regions selectively; this leads to an increase in Na(+) current density and membrane excitability. PMID: 12522174
  45. NHE1 is major regulator of intracellular pH in both resting and muscarinic agonist-stimulated pancreatic acinar cells. PMID: 12842825
  46. Nhe1 plays a role in cardiac ischemia-reperfusion injury. PMID: 12970112
  47. These results suggest that NHE1 is the major pH(i) regulatory mechanism in cortical astrocytes and that ablation of NHE1 in astrocytes attenuates ischemia-induced disruption of ionic regulation and swelling. PMID: 15013953
  48. A global profile of genes regulated in mammalian fibroblasts by decreased pHi induced by impaired activity of the plasma membrane Na-H exchanger NHE1 was characterized by using cDNA microarrays. PMID: 15257760
  49. findings demonstrate a novel role for Na(+)/H(+) exchanger NHE1 in transepithelial HCO(3)(-) absorption in the medullary thick ascending limb PMID: 15292047
  50. ERK1/2 is involved in activation of NHE1 in astrocytes after in vitro ischemia. NHE1-mediated Na+ accumulation subsequently alters Ca2+ homeostasis via Na+/Ca2+ exchange. PMID: 15901600

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Subcellular Location Membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Protein Families Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
Database Links

KEGG: mmu:20544

STRING: 10090.ENSMUSP00000030669

UniGene: Mm.4312

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