Name: Recombinant Mouse Spermine oxidase (Smox)
Brand: CUSABIO
SKU: CSB-EP021844MO

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Smox

Uniprot No.

Q99K82

Research Area

Others

Alternative Names

Smox; SmoSpermine oxidase; EC 1.5.3.16; Polyamine oxidase 1; PAO-1; PAOh1

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-555aa

Target Protein Sequence

MQSCESSGDSADDPLSRGLRRRGQPRVVVIGAGLAGLAAARALLEQGFTDVTVLEASSHIGGRVQSVRLGDTTFELGATWIHGSHGNPIYQLAEANGLLEETTDGERSVGRISLYSKNGVACYLTNRGCRIPKDVVEEFSDLYNEVYNMTQEFFRHGKPVNAESQNSVGVFTREKVRNRIRDDPDDTEATKRLKLAMIQQYLKVESCESSSHSIDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPPHVIQLGKPVRCIHWDQASAHPRGPEIEPRGEGDHNHDTGEGGQSGENPQQGRWDEDEPWPVVVECEDCEVIPADHVIVTVSLGVLKRQYTSFFRPCLPTEKVAAIHRLGIGTTDKIFLEFEEPFWGPECNSLQFVWEDEAESCTLTYPPELWYRKICGFDVLYPPERYGHVLSGWICGEEALVMERCDDEAVAEICTEMLRQFTGNPNIPKPRRILRSAWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTAPMQVLFSGEATHRKYYSTTHGALLSGQREAARLIEMYRDLFQQGP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

77.9kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I have found a paper describing the preparation of mouse recombinant protein of spermine oxidase in E. coli, and they reported the retaining of bioactivity. The paper is attached. Could you please check if you are using a similar approach for purification and isolation? It will give me more confidence to purchase your protein.
Also please send me some informations for the E.coli expressed spermine oxidase.
Have you received any customer feedback regarding reactivity of this protein? Please can you help me to answer the customer about the similarity of the production procedure?

Very nice to receive your inquiry.
Actually, this protein (CSB-EP021844MO) is one of our best-selling products, which has been ordered many times by other customers, and no complaints about it up to now.Since what we provide is the full length protein, and it's purified under very mild condition, so it should be active, however, as we haven't validated, so we can't 100% guarantee its activity.
We also haven't received any feedback about the activity from other customers. I can send you the COA of the previous Lot for your reference.
As we have successfully expressed this protein before, but we didn't use a similar approach for production as introduced in the paper.
If you need us to produce the protein according to the similar approach introduced in the paper, we can choose pet22b or pet26b vector to construct a new vector, these two vectors (pet22b or pet26b) are similar to the pet25b vector that is mentioned in the paper, which contain the pelB signal peptide, the target gene can be localized in the periplasm of the cell during the protein expression. But as we need to constrcut a new expression vector, so the prices and lead time will change.
The culture condition and other purification and isolation methods will be the same as in the paper, but you should remark this requirement when placing the order.

I want to know if there will be an extra fee if I want to remove the N-terminal tag after the purification.

Thanks for your inquiry. Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order.
Sorry, we haven't tried to remove the tag before. We can try enzyme digestion, but we can't guarantee 100% successfully.
The overall success rate of enzyme digestion data analysis is 75%-86%.
* Not all protein tags can be removed as some proteins will be very unstable after tag removal.
* If we succeed in removing the tag, we will charge for extra cost.
* If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
* Generally, the delivery time will be extended for 3 days.

We are interested in your CAT# CSB-EP021844MO.
We are interested in Tag Removal Service.
Please provide estimated lead time for with and without Tag Removal Service.

Very nice to receive your inquiry. Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order.
Sorry, we haven't tried to remove the tag before. We can try enzyme digestion, but we can't guarantee 100% successfully.
The overall success rate of enzyme digestion data analysis is 75%-86%.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.

Target Background

Function

Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.

Gene References into Functions

Novel evidences of the complex and critical functions carried out by spermine oxidase and spermine in the mammalian brain. PMID: 23840306
Spermine oxidase is an important regulator of muscle gene expression and fiber size. PMID: 25406264
Data (including data from studies using recombinant mutant proteins) suggest that Glu216-Ser218 is a hot spot responsible for Smox substrate specificity; Smox with mutation E216L/S218A exhibits specificity for N'-acetylspermine rather than spermine. PMID: 24854736
ROS overexposure by mSMOX delivers an earlier adaptive response to radiation PMID: 23857253
Spermine oxidase mediates the gastric cancer risk associated with Helicobacter pylori CagA. PMID: 21839041
Spermine oxidase binds spermine in a similar conformation as that observed in the yeast polyamine oxidase FMS1-spermine complex and demonstrate a major role for residues His82 and Lys367 in substrate binding and catalysis. PMID: 20839014
expression and characterization of mouse spermine oxidase PMID: 12458219
we identified a second domain that is necessary for nuclear localization of the spermine oxidase splice variant PMID: 15955064

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Subcellular Location

Cytoplasm.; [Isoform 2]: Cytoplasm. Nucleus.

Protein Families

Flavin monoamine oxidase family

Tissue Specificity

Widely expressed. Isoform 1 and isoform 2 are expressed at higher level in brain and skeletal muscle. Isoform 7 is found in brain and spleen, isoform 10 is widely expressed but found at lower level in heart, kidney, liver and lung.

Database Links

KEGG: mmu:228608

UniGene: Mm.136586

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